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Evaluation of the antioxidant activity of passion fruit (Passiflora edulis and Passiflora alata) extracts on stimulated neutrophils and myeloperoxidase activity assays
Authors:Maria Luiza Zeraik  Didier Serteyn  Ginette Deby-Dupont  Jean-Nöel Wauters  Monique Tits  Janete H Yariwake  Luc Angenot  Thierry Franck
Affiliation:1. Institute of Chemistry of São Carlos, University of São Paulo, P.O. Box 780, 13560-970 São Carlos, SP, Brazil;2. Centre for Oxygen Research and Development (C.O.R.D.), Institute of Chemistry B6a, University of Liège, 4000 Liège, Belgium;3. Laboratory of Pharmacognosy, Institute of Pharmacy, University of Liège, CHU, B-4000 Liège 1, Belgium
Abstract:The antioxidant activity of methanol extracts from Passiflora edulis and Passiflora alata pulp, and P. edulis rinds, healthy or infected with the passion fruit woodiness virus (PWV), was investigated using the oxidant activities of the neutrophil and the neutrophil granule enzyme myeloperoxidase (MPO), both playing key roles in inflammation. The reactive oxygen species produced by stimulated neutrophils were evaluated by lucigenin-enhanced chemiluminescence (CL) and the activity of purified MPO was measured by SIEFED (Specific Immunological Extraction Followed by Enzymatic Detection), a technique for studying the direct interaction of a compound with the enzyme. The rind extracts of P. edulis possessed higher and dose-dependent inhibitory effects on CL response and on the peroxidase activity of MPO than total pulp extracts from both passion fruit species. The quantification of isoorientin in the extracts showed a correlation with their antioxidant activity, suggesting the potential of P. edulis rinds as functional food or as a possible source of natural flavonoids.
Keywords:Passion fruit  Passiflora edulis Sims f  flavicarpa Degener  Passiflora alata Dryander  Flavonoids  Isoorientin  Antioxidant activity  Neutrophils  Myeloperoxidase
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