Isolation and characterisation of arabinogalactan-proteins from New Zealand kanuka honey |
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| Authors: | Gregor Steinhorn Ian M. Sims Susan M. Carnachan Alistair J. Carr Ralf Schlothauer |
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| Affiliation: | 1. Comvita New Zealand Limited, Private Bag 1, Te Puke 3153, New Zealand;2. Industrial Research Limited, PO Box 31-310, Lower Hutt 5040, New Zealand;3. Institute of Food Nutrition and Human Health, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand |
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| Abstract: | Fractionation of manuka, kanuka and clover honeys indicated the >10 kDa fraction contained small amounts of type II arabinogalactans (AGs), which are often present as arabinogalactan proteins (AGPs). AGPs were isolated from the >10 kDa fraction of kanuka honey using β-glucosyl Yariv reagent and their composition and structure analysed. Constituent sugar, glycosyl linkage and NMR spectroscopy analysis of the purified AGP fraction revealed a predominance of neutral sugars, mainly galactose and arabinose, linked in a highly-branched structure typical of type II AGs. The molecular weight of the major component of the purified AGPs was ∼110 kDa, as determined by size-exclusion chromatography-multi-angle laser light scattering (SEC-MALLS). The Yariv supernatant fraction contained less total sugar, especially galactose, and more protein than purified AGPs. Linkage analysis indicated this fraction also contained an AG-type polymer in addition to various other polysaccharides and SEC-MALLS indicated the molecular weight of the major component was ∼32 kDa. |
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| Keywords: | Honey Kanuka Arabinogalactan-protein Structural analysis NMR |
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