High throughput analysis of amnesic shellfish poisoning toxins in bivalve molluscs by dispersive solid-phase extraction and high-performance liquid chromatography using a monolithic column |
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| Authors: | Jorge Regueiro,Gonzalo Á lvarez,Aida Mauriz,Juan Blanco |
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| Affiliation: | 1. Centro de Investigacións Mariñas (Xunta de Galicia), Apartado 13, E-36620 Vilanova de Arousa, Pontevedra, Spain;2. Facultad de Ciencias del Mar, Departamento de Acuicultura, Universidad Católica del Norte, Larrondo 1281, Coquimbo, Chile |
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| Abstract: | Domoic acid and its isomers are algal neurotoxins responsible for the human intoxication syndrome known as amnesic shellfish poisoning. A rapid and simple HPLC–UV/DAD method based on the use of a monolithic silica column is proposed for the determination of ASP toxins in shellfish samples. To the best of our knowledge, a monolithic column is applied for the first time to the analysis of marine toxins. |
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| Keywords: | Amnesic shellfish poisoning Domoic acid Dispersive solid-phase extraction HPLC Monolithic column Experimental design |
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