Validation of a UHPLC-FLD method for the simultaneous quantification of aflatoxins,ochratoxin A and zearalenone in barley |
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| Authors: | María Ibáñez-Vea Laura Ana Corcuera Rebeca Remiro María Teresa Murillo-Arbizu Elena González-Peñas Elena Lizarraga |
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| Affiliation: | 1. Organic and Pharmaceutical Chemistry Department, Faculty of Pharmacy, C.I.F.A., University of Navarra, C/ Irunlarrea 1, 31008 Pamplona, Navarra, Spain;2. Nutrition and Food Sciences, Physiology and Toxicology Department, Faculty of Pharmacy, C.I.F.A., University of Navarra, C/ Irunlarrea 1, 31008 Pamplona, Navarra, Spain |
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| Abstract: | A fast and simple UHPLC-FLD method has been developed for the simultaneous determination in barley of aflatoxins (B1, G1, B2 and G2), ochratoxin A (OTA) and zearalenone (ZEA), some of the most important mycotoxins due to their toxicity and occurrence. The procedure is based on the extraction of the six mycotoxins with a mixture of acetonitrile and water, and the purification of the extract with immunoaffinity columns before analysis. Detection of AFB1 and AFG1 is improved using a photochemical reaction. The method has been validated with satisfactory results. Limits of detection were 340 ng kg−1 for ZEA, 13 ng kg−1 for OTA and varied from 0.5 to 15 ng kg−1 for aflatoxins. Recovery percentages were between 78.2% and 109.2%. After being validated, the method has been successfully applied to 20 barley samples cultivated in a region of northern Spain (Navarra). |
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| Keywords: | Mycotoxin Aflatoxins Ochratoxin A Zearalenone UHPLC-FLD Barley |
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