Stratum Corneum Lipid Structure Investigated by EPR Spin-Probe Method: Application of Terpenes |
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| Authors: | Kouichi Nakagawa Kazunori Anzai |
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| Affiliation: | (1) RI Research Center, Fukushima Medical University, 1 Hikarigaoka, Fukushima 960-1295, Japan;(2) Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4-9-1 Anagawa, Chiba 263-8555, Japan;(3) Nihon Pharmaceutical University, 10281 Komuro, Ina-machi, Kitaadachi-gun Saitama, 362-0806, Japan;(4) Division of Special Research, National Institute of Radiological Sciences, 4-9-1 Anagawa, Chiba 263-8555, Japan |
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| Abstract: | Electron paramagnetic resonance (EPR) in conjunction with a slow-tumbling simulation was utilized for defining stratum corneum
(SC) lipid structure. SC from the back of hairless mouse (HOS:HR-1) was stripped consecutively from one to three or four times
using a glass plate coated with a cyanoacrylate resin. Aliphatic spin probes, 5-doxylstearic acid (5-DSA) and 3β-doxyl-5α-cholestane
(CHL), were used to evaluate the SC ordering. EPR spectrum of 5-DSA incorporated in the SC demonstrated a characteristic peak
for the first strip. A slow-tumbling simulation for 5-DSA showed clear differences in EPR intensities as well as ordering
values (S
0) of the SC for control and terpenes treated SC. The α-terpineol enhanced the permeation of the single chain 5-DSA about three
times more than that of the control. However, EPR spectra of CHL in the SC did not show a clear difference for each strip,
except for the signal intensity. The results imply that CHL permeates into SC lipid differently from 5-DSA. The enhancement
of the 5-DSA is more significant than that of CHL. Therefore, the present results can be useful for various drug administrations
via the skin. |
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