Rapid detection of pork meat freshness by using L-cysteine-modified gold electrode |
| |
Authors: | Liliane Clarisse Umuhumuza Xiulan Sun |
| |
Affiliation: | (1) State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, 214122 Wuxi, Jiangsu Province, People’s Republic of China |
| |
Abstract: | In this research, a simple and rapid glucose sensor was developed and applied in the detection of meat glucose. The gold electrode
was modified using L-cysteine and nanogold solution, and a polyglutamate–glucose oxidase (GOx) complex was prepared and dropped
on the modified electrode; the electrostatic binding between the L-cysteine on the electrode surface and the polyglutamate
moiety of the complex resulted in the formation of a GOx-attached electrode. Glucose in meat was detected, and the enzyme
electrode showed a quick response. The optimum operating conditions for this sensor were determined as follows: 0.1 M citrate
buffer pH 5.0 was found to be suitable for this experiment, and the temperature was kept between 25 and 30 °C which is the
suitable range for the GOx activity. A linear relationship was found between 0.1 and 0.9 mM/mL. High pressure liquid chromatography
(HPLC) was also used to detect pork meat glucose, and total volatile basic nitrogen (TVBN) production was as well detected
as a quality index of pork meat freshness. The results showed that the developed sensor was fast and reliable method for the
detection of glucose in meat. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|