| BAPTA-AM对OGD-再灌致HepG-2细胞损伤的保护作用研究 |
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| 引用本文： | 宋必卫,骆钱芬.BAPTA-AM对OGD-再灌致HepG-2细胞损伤的保护作用研究[J].浙江工业大学学报,2013,41(2):133-137,170. |
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| 作者姓名： | 宋必卫 骆钱芬 |
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| 作者单位： | 浙江工业大学药学院,浙江杭州,310032 |
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| 摘 要： | 观察BAPTA-AM抗OGD-再灌对HepG-2细胞的损伤作用及其机制.在氧/糖剥夺环境中培养细胞12h后复糖复氧,检测培养液中乳酸脱氢酶(LDH)、谷丙转氨酶(ALT)及谷草转氨酶(AST)的活性,MTT法检测细胞活力,Annexin V-EGFP/PI荧光染色检测细胞死亡率,A23187拮抗实验及Fura-2/AM测定细胞内游离钙浓度等方法,评价BAPTA-AM抗氧/糖剥夺-再灌注致HepG-2细胞损伤的作用.BAPTA-AM能明显抑制OGD-再灌引起的HepG-2细胞培养液中的LDH、ALT、AST水平的升高,抑制HepG-2细胞的坏死和细胞内游离钙浓度的升高,明显提高HepG-2细胞活力,A23187减弱BAPTA-AM保肝作用.BAPTA-AM通过降低细胞内游离钙浓度,明显减轻OGD-再灌对HepG-2细胞的损伤.
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| 关 键 词： | BAPTA-AM HepG-2细胞 OGD-再灌 |
Protection of HepG-2 cell of BAPTA-AM against deprivation of O2 and glucose/reperfusion-induced injury |
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| SONG Bi-wei
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LUO Qian-fen.Protection of HepG-2 cell of BAPTA-AM against deprivation of O2 and glucose/reperfusion-induced injury[J].Journal of Zhejiang University of Technology,2013,41(2):133-137,170. |
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| Authors: | SONG Bi-wei LUO Qian-fen |
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| Affiliation: | (College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou 310032,China) |
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