Enhanced stability of Candida antarctica lipase B in ionic liquids |
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| Authors: | Sung Ho Ha Sang Hyun Lee Dung Thanh Dang Min Sik Kwon Woo-Jin Chang Yong Jae Yu Il Suk Byun Yoon-Mo Koo |
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| Affiliation: | (1) ERC for Advanced Bioseparation Technology, Inha University, Incheon, 402-751, Korea;(2) Department of Biological Engineering, Inha University, Incheon, 402-751, Korea;(3) C-TRI, Hwaseong-city, Gyeonggi-do, 445-743, Korea |
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| Abstract: | The activity and stability of lipase from Candida antarctica were investigated in the kinetic resolution of (R,S)-1-phenylethanol with vinyl acetate using ionic liquids (ILs) as reaction media. Among ILs tested, the highest activity of lipase was observed in [Edmim][Tf2N]. In hydrophobic ILs such as [Edmim][Tf2N], [Emim][Tf2N] and [Pmim] [PF6], lipase could retain its activity after 5 times reuse, while the activity of lipase in hydrophilic ILs and organic solvents was drastically decreased. The activities of lipase in [Edmim][Tf2N], [Emim][Tf2N] and [Pmim][PF6] were also well maintained after 1 day incubation at 80 °C. The lipase suspended in [Edmim][Tf2N] could be successfully reused 6 times without loss of activity. |
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| Keywords: | Ionic Liquid Lipase (R,S)-1-Phenylethanol Reuse Stability |
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