| 利用酶抑制剂固定化磷脂酶A1（PLA1）的研究 |
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| 引用本文： | 孙万成,宋龄英,蒋笃孝,罗毅皓. 利用酶抑制剂固定化磷脂酶A1（PLA1）的研究[J]. 食品科学, 2004, 25(6): 52-56 |
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| 作者姓名： | 孙万成 宋龄英 蒋笃孝 罗毅皓 |
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| 作者单位： | 暨南大学食品科学与工程系，广州大学化学系，青海大学农牧学院 |
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| 基金项目： | 国务院侨办重点资助项目(93A119) |
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| 摘 要： | 在固定化磷脂酶A1时,利用酶抑制剂与磷脂酶A1活性中心氨基酸残基结合,使戊二醛基与磷脂酶A1的非活性氨基残基缩合,从而起到保护酶活性中心基团,提高固定化酶活力作用。实验证明磷酸盐是磷脂酶A1的竞争性抑制剂,并求出Ki值。
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| 关 键 词： | 酶抑制剂 固定化磷脂酶A1 |
| 文章编号： | 1002-6630(2004)06-0052-05 |
| 修稿时间： | 2003-09-12 |
Inhibitor Study on Immobilized PLA1 |
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| SUN Wan-cheng,SONG Ling-ying,JIANG Du-xiao,LUO Yi-hao. Inhibitor Study on Immobilized PLA1[J]. Food Science, 2004, 25(6): 52-56 |
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| Authors: | SUN Wan-cheng SONG Ling-ying JIANG Du-xiao LUO Yi-hao |
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| Affiliation: | 1.Food Science and Technology Department,Jinan University;2.Chemistry Department,Guangzhou University;3.College of Agriculture and Animal Husbandry,Qinghai University |
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| Abstract: | When immobilized PLA1, inhibitor can couple amine residues which are important for catalysis or maintaining proteinstructure. Therefore, it can protect amine groups of activating site by binding between glutaradehyde and inactive groups of amineso as to increase activity retention of immobilized enzyme.In addtion, phosphate is shown to be a competive inhibitor of PLA1,and by means of making chart to get the Ki value. |
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| Keywords: | inhibitor immobilized PLA1(phospholipase A1) |
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