应用免疫印迹法分析丙型肝炎相关性抗体

应用两种免疫印迹法对二代ELISA测试的69份血清进行了追加试验。结果表明:8份ELISA阴性血清免疫印迹均阴性,61份ELISA阳性血清有1份免疫印迹阴性。ELISA假阳性率为1.67％。在60份免疫印迹阳性血清中,抗结构区(capsid),非结构区NS_3和NS_4抗体的阳性率分别为83％、93.3％和70％。其中丙型肝炎相关性抗体的分布,以抗capsid、NS_3、NS_4抗体都阳性为主(58.7％)。在输血后急性肝炎和肝细胞癌患者中。丙型肝炎相关性抗体的分布没有统计学上的差异。

Analysis of Hepatitis C Associated Antibody Immunoblot Assay

western blot and Recombinant immunoblot assays were used to detect anti-HCV antibodies in 69 sera which had been detected by the second-generation ELISA. Of the 69 sera, all the 8 ELISA negative sera were confirmed anti-HCV negative, but one of the 61 ELISA positive sera was found to be anti-HCV false positive by immunoblot assay. The false positive rate of the ELISA assay for detection of HCV antibody Was 1.67%. Of the 60 true positive sera, the positive rates of antibodies to capsid, NS_3 and NS_4 recombinant antigens were 83%, 93% and 70%, respectively, and all the antibodies to capsid, NS_3 and NS_4 antigens were positive in more than half of the 60 sera (58%). It had no significant statistical difference between the prevalence of HCV associated antibodies of the patients with hepatocellular carcinoma and post-transfusion acute hepatitis."