| 降糖类中成药中非法添加化学成分的测定 |
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| 引用本文： | 付大友,陈祥辉,谭文渊,王绍楠,张小芳.降糖类中成药中非法添加化学成分的测定[J].现代化工,2012(1):94-96. |
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| 作者姓名： | 付大友 陈祥辉 谭文渊 王绍楠 张小芳 |
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| 作者单位： | 四川理工学院材料与化学工程学院 |
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| 基金项目： | 四川理工学院研究生创新基金;四川省青年基金(Y2010022、11ZB253) |
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| 摘 要： | 通过超声-微波协同萃取仪预处理样品,建立了降糖类中成药中非法添加的盐酸二甲双胍、格列苯脲、格列吡嗪3种化学物质同时分离测定的高效液相色谱方法。采用ZORBAX Eclipse XDB-C18色谱柱,以V(0.1 mol/L磷酸二氢钠缓冲液)∶V(甲醇)为30∶70作流动相,流速为1.0 mL/min,检测波长为233 nm,柱温为30℃,进样量为20μL。该方法快速、灵敏度高、重现性好,适用于大批量样品的快速鉴别。
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| 关 键 词： | 降糖类 中成药 非法 添加 |
Determination of chemicals mixed illegally in anti-hypoglycemic Chinese patent medicine |
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| FU Da-you,CHEN Xiang-hui,TAN Wen-yuan,WANG Shao-nan,ZHANG Xiao-fang.Determination of chemicals mixed illegally in anti-hypoglycemic Chinese patent medicine[J].Modern Chemical Industry,2012(1):94-96. |
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| Authors: | FU Da-you CHEN Xiang-hui TAN Wen-yuan WANG Shao-nan ZHANG Xiao-fang |
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| Affiliation: | (Material and Chemical Engineering College,Sichuan University of Science and Engineering,Zigong 643000,China) |
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| Abstract: | In this paper,the HPLC method of separating and determining three kinds of chemical compounds,met-formin hydrochloride,glibenclamide and glipizide,which were mixed illegally in antidiabetic traditional Chinese medicine,was established after the pretreatment of sample by ultra sonic-microwave-assisted extractor.The details are shown as follows:ZORBAX Eclipse XDB-C18 column,mobile phase of 0.1 mol/L sodiumydrogen phosphate buffer solution-methanol(30∶70 of volume ratio),1 mL/min of flow rate,233 nm of detection wave length,30℃ of column temperature and 20 μL of the volume of injection.The method is rapid,highly sensitive and reappearing consistently.Therefore,it is suitable for the rapid identification of the multitudinous samples. |
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| Keywords: | hypoglycesmic Chinese patent medicine illegal addition |
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