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Effect of substrate concentration on fermentative hydrogen production from sweet sorghum extract |
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| Authors: | Georgia Antonopoulou Hariklia N. Gavala Ioannis V. Skiadas Gerasimos Lyberatos |
| Affiliation: | a Department of Chemical Engineering, University of Patras, 1 Karatheodori St., GR 26500 Patras, Greece b Institute of Chemical Engineering and High Temperature Chemical Processes, GR 26504 Patras, Greece c Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University Copenhagen (AAU Cph), Lautrupvang 15, DK 2750 Ballerup, Denmark |
| Abstract: | The aim of the present study was to assess the influence of substrate concentration on the fermentative hydrogen production from sweet sorghum extract, in a continuous stirred tank bioreactor. The reactor was operated at a Hydraulic Retention Time (HRT) of 12 h and carbohydrate concentrations ranging from 9.89 to 20.99 g/L, in glucose equivalents. The maximum hydrogen production rate and yield were obtained at the concentration of 17.50 g carbohydrates/L and were 2.93 ± 0.09 L H2/L reactor/d and 0.74 ± 0.02 mol H2/mol glucose consumed, corresponding to 8.81 ± 0.02 L H2/kg sweet sorghum, respectively. The main metabolic product at all steady states was butyric acid, while ethanol production was high at high substrate concentrations. The experiments showed that hydrogen productivity depends significantly on the initial carbohydrate concentration, which also influences the distribution of the metabolic products. |
| Keywords: | Sweet sorghum Hydrogen Substrate concentration Continuous fermentation Biohydrogen Fermentative hydrogen production |
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