| 丙酮酸高产菌诱变选育及发酵工艺优化的初步研究 |
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| 引用本文： | 胡浩然,石勇,陈雄.丙酮酸高产菌诱变选育及发酵工艺优化的初步研究[J].化学与生物工程,2011,28(2):59-62. |
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| 作者姓名： | 胡浩然 石勇 陈雄 |
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| 作者单位： | 湖北工业大学生物工程学院,发酵工程省部共建教育部重点实验室,湖北,武汉,430068 |
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| 基金项目： | 湖北工业大学发酵工程省部共建教育部重点实验室2009年开放基金课题(2009KFJJ07) |
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| 摘 要： | 以光滑球拟酵母(Torulopsis glabrata)HB20为出发菌,经过UV和DES复合诱变选育出一株丙酮酸高产菌HB304.鉴定其为维生素营养缺陷型,摇瓶发酵产酸量比原菌增加了31.2%;优化培养基后其摇瓶发酵产酸量比原菌增加了41.9%.
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| 关 键 词： | 丙酮酸 复合诱变 育种 优化 |
Mutation Breeding and Preliminary Optimization of the Fermentation Process for Pyruvic Acid-Producing Strain |
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| HU Hao-ran,SHI Yong,CHEN Xiong.Mutation Breeding and Preliminary Optimization of the Fermentation Process for Pyruvic Acid-Producing Strain[J].Chemistry & Bioengineering,2011,28(2):59-62. |
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| Authors: | HU Hao-ran SHI Yong CHEN Xiong |
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| Affiliation: | HU Hao-ran,SHI Yong,CHEN Xiong(Key Laboratory of Fermentation Engineering of Ministry of Education,Hubei University of Technology,Wuhan 430068,China) |
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| Abstract: | A high pyruvic acid-producing strain HB304 was bred from Torulopsis glabrata HB20 by UV-DES compound mutation.The strain HB304 appeared to be auxotroph.The yield of pyruvic acid was increased by 31.2% than that of the wild strain in flask culture.After optimization about the medium,the yield of pyruvic acid was increased by 41.9% than that of the wild strain in flask culture. |
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| Keywords: | pyruvic acid compound mutation breeding optimization |
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