| 核酸适配体在金黄色葡萄球菌检测中的应用进展 |
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| 引用本文： | 崔妍,白亚龙,史贤明.核酸适配体在金黄色葡萄球菌检测中的应用进展[J].食品工业科技,2021,0(21):1-7. |
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| 作者姓名： | 崔妍 白亚龙 史贤明 |
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| 作者单位： | 1.上海交通大学农业与生物学院，中美食品安全联合研究中心，微生物代谢国家重点实验室，上海 2002402.上海市农业科学院农产品质量标准与检测技术研究所，上海 201403 |
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| 基金项目： | 国家重点研发计划政府间国际科技创新合作项目（2019YFE0119700）；2019年上海交通大学决策咨询课题实验技术专项（JCZXSJB2019-013） |
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| 摘 要： | 金黄色葡萄球菌（Staphylococcus aureus）是一种重要的食源性致病菌，快速检测方法的开发对于防控该菌引发的食源性疾病具有重要意义。生物识别物是快速检测的核心，核酸适配体作为新兴的生物识别物，与靶标分子有高度的亲和力及特异性，且易于人工合成和修饰，在食源性致病菌的检测方面具有较大的潜力和优势。本文综述了核酸适配体的筛选技术及其在金黄色葡萄球菌分离富集和快速检测中的应用进展，以期为食源性致病菌新型检测方法的开发和实际应用拓宽思路。
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| 关 键 词： | 核酸适配体 金黄色葡萄球菌 筛选 检测 生物传感器 |
| 收稿时间： | 2021-06-17 |
Progress on the Application of Aptamers in the Detection of Staphylococcus aureus |
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| CUI Yan,BAI Yalong,SHI Xianming.Progress on the Application of Aptamers in the Detection of Staphylococcus aureus[J].Science and Technology of Food Industry,2021,0(21):1-7. |
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| Authors: | CUI Yan BAI Yalong SHI Xianming |
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| Affiliation: | 1.School of Agriculture and Biology, Shanghai Jiao Tong University, MOST-USDA Joint Research Center for Food Safety, State Key Lab of Microbial Metabolism, Shanghai 200240, China2.Institute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China |
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| Abstract: | Staphylococcus aureus is one of the most important foodborne pathogens. Rapid detection is of vital importance for the prevention and control of outbreaks caused by this bacterial pathogen. Biological recognition elements are the core of rapid detection. As the emerging biological recognition element, nucleic acid aptamers are easy to be prepared and modified, and have high affinity and specificity that can be combined with targets well, which have great potential and advantages when applied in the detection of foodborne pathogens. For the sake of broadening research ideas on development and application of detection methods for Staphylococcus aureus, aptamer screening technologies and detection methods based on aptamers are reviewed in this article. |
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