漆酶催化二氢杨梅素氧化聚合

系统考察漆酶催化条件对二氢杨梅素衍生物的颜色、α-葡萄糖苷酶抑制及抗氧化活性的影响,并运用波谱手段对产物结构进行表征.结果表明漆酶可用于二氢杨梅素的氧化聚合,且漆酶浓度、pH值、反应时间及温度对衍生物的颜色有规律性影响.所得二氢杨梅素衍生物的α-葡萄糖苷酶抑制活性(IC506.2μg/mL)显著高于二氢杨梅素(IC50...

Oxidative Polymerization of Dihydromyricetin Catalyzed by Laccase

The effects of laccase catalysis conditions on the color, α-glucosidase inhibitory activity and antioxidant activity of dihydromyricetin derivatives were systematically investigated, and the structures of the derivatives were also characterized. It was found that the oxidative polymerization of dihydromyricetin was achieved by using laccase. Laccase concentration, pH, reaction time and temperature had regular effects on the color of the derivatives. The α-glucosidase inhibitory activity of the derivatives was significantly higher than that of dihydromyricetin, with half-maximal inhibitory concentration (IC50) of 6.2 versus 85.95 μg/mL, but the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical cation scavenging capacities were inferior to those of dihydromyricetin. Spectral analysis indicated that laccase catalyzed the oxidation of the phenolic hydroxyl groups of dihydromyricetin, consequently leading to the polymerization of dihydromyricetin by ether bonding. These results can promote the application of dihydromyricetin in foods, and the development of new functional foods.