低聚果糖对C57BL/6小鼠肥胖预防及其肠道菌群的调节作用

为探究低聚果糖（fructooligosaccharides,FOS）对C57BL/6小鼠肥胖预防及其肠道菌群的调节作用，将50只雄性5周龄C57BL/6小鼠随机分为5组，正常组食用正常饲料，其他组通过食用高脂饲料构建肥胖模型，正常组、模型组灌胃给予生理盐水，实验组灌胃给予高、低剂量的FOS，阳性对照组灌胃给予奥利司他，实验进行6周。采用高通量16S rRNA基因扩增测序、酶联免疫吸附测试等方法，分析FOS对C57BL/6小鼠的肥胖预防及肠道菌群的调节作用。结果表明，高、低剂量FOS都显著降低了肥胖模型小鼠的Lee’s指数、附睾脂肪指数（P<0.05），并显著升高了肥胖模型小鼠血清高密度脂蛋白胆固醇（high density lipoprotein cholesterol,HDL-C）浓度（P<0.05）；高剂量FOS显著降低了肥胖模型小鼠的体质量增量及血清低密度脂蛋白胆固醇（low density lipoprotein cholesterol,LDL-C）、肿瘤坏死因子α(tumor necrosis factor α,TNF-α)、脂多糖（lipopolysaccha...

Dietary Fructooligosaccharides Prevent Obesity and Regulate the Gut Microbiota in C57BL/6 Mice

To investigate the effect of dietary fructooligosaccharides (FOS) in preventing obesity and regulating the gut?microbiota in C57BL/6 mice, 50 male five-week-old C57BL/6 mice were randomly divided into five groups. One group received a normal diet as a control group, while the other four groups were fed a high fat diet to induce obesity. The mice in the normal and model groups were given normal saline intragastrically, those in the experimental groups were given high and low doses of FOS intragastrically, and those in the positive control group were given orlistat intragastrically. The experiment lasted for six weeks. High-throughput 16S rRNA gene amplicon sequencing and enzyme-linked immunosorbent assay (ELISA) were carried out in this study. The results showed that both high- and low-dose FOS significantly reduced the Lee’s index and epididymal?adipose index (P < 0.05), and significantly increased the high density lipoprotein cholesterol (HDL-C) level of the obese mouse model (P < 0.05). High-dose FOS significantly reduced the body mass gain, and the levels of serum low density lipoprotein cholesterol (LDL-C), tumor necrosis factor α (TNF-α) and lipopolysaccharide (LPS) in the obese mouse model. The gut microbiota analysis showed that compared with the normal group, the relative abundance of Firmicutes in the model group was significantly increased (P < 0.05), and the relative abundance of Bacteroidota was significantly decreased (P < 0.05). The gut microbiota of mice in the FOS intervention group was restored to a certain extent, and the composition of gut microbiota was close to that of the normal group. Therefore, FOS can control obesity and improve the gut microbiota in mice.