基于膜技术的灵芝粗多糖分级分离及抗氧化活性比较

本文主要研究膜分离技术对灵芝粗多糖分级分离效果及其抗氧化活性的影响.以灵芝子实体为原料,通过热水浸提后用100、10和1 kDa超滤膜多级组合对灵芝粗多糖进行分级分离,分别记为GLP100、GLP10和GLP1.比较3种不同孔径膜对灵芝粗多糖的分级效果、理化性质和抗氧化活性的差异.傅里叶红外变换光谱分析结果证明3种多糖...

Fractionation and Antioxidant of Crude Polysaccharide from Ganoderma lucidum Based on Membrane Technology

Effects of membrane technology on the separation of crude polysaccharides from Ganoderma lucidum and its antioxidant activity had been studied. Fruiting body of Ganoderma lucidumwas leached by hot water, 100, 10 and 1 kDa ultrafiltration membrane were used to classify these crude polysaccharides recorded as GLP100, GLP10 and GLP1. Physicochemical properties and antioxidant activity of crude polysaccharides were compared. It had been demonstrated that three polysaccharides all have typical β-glucosidic bond absorption peaks by FTIR analysis. Congo red and circular dichroism analysis showed that the three crude polysaccharides were helix polysaccharides, GLP100 and GLP10 were triple helix polysaccharides. Results of SEM showed that the sizes of the three polysaccharides were different, which further verified that the different membranes could be used to grade the Ganoderma lucidum polysaccharides. All the three crude polysaccharides showed certain antioxidant capacity but some differences by reducing power, OH, 2, 2'-diazobis(3-ethylbenzothiazolin-6-sulfonic acid) diammonium salt cationic radical, 1, 1-diphenyl-2-trinitrobenzene hydrazine free radical scavenging. Among them, the reducing power of GLP100 was very close, and the scavenging ability of OH, ABTS and DPPH free radicals of GLP100 was better than that of the other two polysaccharides, The ABTS radical scavenging ability of GLP1 was better than the other two polysaccharides. Results showed that multistage membrane separation technology could effectively classify the polysaccharides of Ganoderma lucidum.