芹菜苷对葡聚糖硫酸钠诱导小鼠结肠炎的缓解作用

目的：借助葡聚糖硫酸钠（dextran sulfate sodium，DSS）诱导的结肠炎小鼠模型，探究芹菜苷对肠道健康的影响。方法：将30 只雄性C57BL/6J小鼠随机分成正常组、模型组、芹菜苷组。正常组和模型组全程给予AIN93G纯饲料，芹菜苷组全程给予基于AIN93G纯饲料配方调整的芹菜苷（质量分数0.016 7%）定制饲料，饲喂7 d后，模型组和芹菜苷组的饮用水更换为含质量分数3% DSS的无菌水进行肠炎造模，造模6 d后，停止给予DSS无菌水，24 h后处死小鼠；每天观察并记录小鼠饮食量、体质量，计算小鼠疾病活动指数（disease activity index，DAI），在实验结束后测定小鼠脾脏指数，血清中肿瘤坏死因子（tumor necrosis factor，TNF）-α、白细胞介素（interleukin，IL）-6水平及结肠组织中髓过氧化物酶（myeloperoxidase，MPO）、诱导型一氧化氮合酶（inducable nitric oxide synthase，iNOS）活力，观察小鼠结肠病理及杯状细胞的数量状况。结果：与模型组相比，芹菜苷可显著缓解结肠炎小鼠的体质量下降（P＜0.05），降低DAI（P＜0.001）与脾脏指数（P＜0.001）以及血清中炎症因子TNF-α（P＜0.001）与IL-6（P＜0.01）的质量浓度；另外，芹菜苷对结肠缩短有极显著改善作用（P＜0.01），并可减轻结肠炎小鼠结肠组织损伤和降低炎症浸润水平，极显著降低MPO及iNOS活力（P＜0.001），显著增加杯状细胞的数量（P＜0.05），促进黏液分泌，维持肠道屏障功能完整。结论：25 mg/kg mb的芹菜苷剂量可缓解DSS诱导的小鼠结肠炎。

Apiin Alleviates Colitis Induced by Dextran Sulfate Sodium in Mice

Objective: To investigate the effect of apiin on intestinal health in mice with colitis induced by dextran sodium sulfate (DSS). Methods: Thrity male C57BL/6J mice were randomly divided into normal, model and apiin groups. The normal and model group were given the AIN93G diet alone, and the apiin group was given the same diet supplemented with apiin (0.016 7%) throughout the whole course. After feeding for seven days, the drinking water of the model and apiin groups was switched to sterile water containing 3% DSS to induce colitis. After six days of treatment, DSS was stopped, and the mice were sacrificed 24 hours later. Food intake and body mass of the mice were observed and recorded every day, and disease activity index (DAI) and spleen index were calculated at the end of the experiment. The levels of tumor necrosis factor (TNF)-α and interleukin (IL)-6 in serum and the activities of myeloperoxidase (MPO) and inducable nitric oxide synthase (iNOS) in colonic tissues were determined. The colonic pathology and the number of goblet cells were observed. Results: Apiin significantly alleviated body mass loss (P < 0.05), decreased DAI (P < 0.001) and spleen index (P < 0.001), and reduced the contents of the inflammatory cytokines TNF-α (P < 0.001) and IL-6 (P < 0.01) in the serum of mice with colitis. In addition, apiin significantly improved colon shortening (P < 0.01), and amelioratesd colonic tissue injury and inflammatory infiltration in mice with colitis. Apiin also significantly decreased the activity of MPO (P < 0.001) and iNOS (P < 0.001), significantly increased the number of goblet cells (P < 0.05), promoted mucus secretion, and maintained the integrity of intestinal barrier function. Conclusion: A dose of 25 mg/kg mb of apiin could alleviate DSS-induced colitis in mice.