高效液相色谱法测定番茄酱中番茄红素含量及其与色度值的相关性

建立基于高效液相色谱法（high performance liquid chromatography, HPLC）的番茄酱中番茄红素异构体及总含量测定方法，并进行了方法学验证。同时，检测并分析了市售番茄制品中番茄红素顺反式异构体的分布情况，探讨了市售番茄制品的色度值与番茄红素含量之间的相关性，方法采用YMC-carotenoid（C₃₀，250 mm×4.6 mm，5 μm）色谱柱、以甲醇-甲基叔丁基醚作为流动相、流速1.3 mL/min进行梯度洗脱，在472 nm检测波长下进行检测，结果表明番茄红素在10~200 μg/mL范围内与峰面积线性关系良好（R2=0.9997），平均加标回收率在98.3%~99.6%之间，相对标准偏差在2.8%~4.5%之间，该方法简便、灵敏、准确，适用于番茄酱中总番茄红素的检测。相关性结果表明色度值a*、Chroma值与番茄红素有极显著的相关性（P<0.01），a*/b*、H°值与番茄红素含量有显著相关性（P<0.05），回归分析的最佳拟合结果为R2=0.689。

Determination of Lycopence in Tomato Products by High Performance Liquid Chromatography and the Correlation with Chromaticity Values

Anvalidated analytical method based on High Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD) was established for the determination of lycopene and its isomers in ketchup. The correlation between the color variables and lycopene content of tomato products from different manufacturers was also discussed. Lycopene was separated by using YMC-carotenoid (C₃₀, 250 mm×4.6 mm, 5 μm) column. Mobile phase contained methanol (A) and methyl tert-butyl ether (B) with flow rate of 1.3 mL/min and was monitored at 472 nm by using DAD. Content of lycopene had a good linear relationship in the concentration range of 10~200 μg/mL (R2=0.9997). The average recoveries ranged from 98.3%~99.6%.The accuracy, expressed in terms of relative standard deviation (RSD), ranged from 2.8% to 4.5%. The method was simple, sensitive, accurate and suitable for the detection of total lycopene in ketchup. The distribution of (all-E)-lycopene and Z-isomers in tomato products was detected and analyzed. The correlation analysis indicated that a*, chroma, a*/b*, hue had remarkable or extremely remarkable correlation with the content of lycopene, the optimal fitting result of regression was R2=0.689.