可口革囊星虫体腔液多糖碱提工艺优化及其抗氧化性研究

为提高可口革囊星虫体腔液利用率，探究可口革囊星虫体腔液多糖的最佳提取工艺及其体外抗氧化特性。采用碱浸提法提取可口革囊星虫体腔液多糖，正交试验法优化多糖提取工艺。以总还原力、1, 1-二苯基-2-三硝基苯肼（DPPH）清除率和羟基自由基清除率为指标测定其抗氧化性，并利用高效液相色谱测定单糖组成，红外光谱对多糖成分进行初步分析。结果表明可口革囊星虫体腔液多糖提取最佳工艺条件为提取温度50 ℃、碱提时间3 h、NaOH质量浓度1.5%，在此工艺下多糖得率为0.92%。该多糖的总还原力、DPPH清除率和羟基自由基清除率的半数清除浓度（IC₅₀）分别为2.976、0.567和0.605 mg/mL。高效液相色谱显示该多糖主要由葡萄糖组成。红外光谱结果显示该多糖是一种含有乙酰氨基和吡喃环，以α-糖苷键连接的多糖。由此可知，可口革囊星虫体腔液多糖有较好抗氧化性，具有良好的应用前景。

Study on Alkaline Extraction Process Optimization of Polysaccharides from Coelomic Fluid of Phasolosma esculenta and Its Antioxidant Activity in vitro

In order to improve the utilization rate of the coelomic fluid of Phascoloma esculenta, the optimal extraction technology and its antioxidant properties in vitro were explored. The polysaccharide of Phascoloma esculenta was extracted by alkali extraction method and optimized by orthogonal experiment. The total reducing power, DPPH scavenging rate and hydroxyl radical scavenging rate were used to determine its antioxidant properties. The composition of monosaccharides was determined by high performance liquid chromatography, and the polysaccharide composition was analyzed by infrared spectroscopy. The results showed that the optimal extraction process conditions for polysaccharides from the coelomic fluid of Phascoloma esculenta were extraction temperature of 50 ℃, alkaline extraction time of 3 h, and NaOH concentration of 1.5%. The yield of polysaccharides under this process was 0.92%. The half scavenging concentration (IC₅₀) of the total reducing power, DPPH scavenging rate and hydroxyl radical scavenging rate were 2.976, 0.567 and 0.605 mg/mL, respectively. The results of HPLC showed that the polysaccharide was mainly composed of glucose and infrared spectroscopy showed that the polysaccharide contained acetylamino and pyran rings and connected by α-glycosidic bonds. It could be seen that the polysaccharides from the coelomic fluid of Phascoloma esculenta have good antioxidant properties and application prospect.