淫羊藿骨碎补联合骨营养补剂对骨质疏松大鼠的影响

目的:研究淫羊藿骨碎补鹿骨粉联合骨营养补剂对去卵巢所致骨质疏松大鼠的影响。方法:制备去卵巢大鼠，随机分为模型组、阳性药组（1.0 mg/kg·bw戊酸雌二醇片）、中药组（0.17 g/kg·bw淫羊藿骨碎补提取物，0.08 g/kg·bw鹿骨粉）、营养剂组（0.17 g/kg·bw氨基葡萄糖盐酸盐，0.08 g/kg·bw硫酸软骨素，0.17 g/kg·bw碳酸钙）、复方组（0.17 g/kg·bw淫羊藿骨碎补提取物，0.08 g/kg·bw鹿骨粉，0.17 g/kg·bw氨基葡萄糖盐酸盐，0.08 g/kg·bw硫酸软骨素，0.17 g/kg·bw碳酸钙），另设假手术组。以上各组灌胃体积均为10 mL/kg·bw，1次/d，连续灌胃12周，每周称量体重并按体重调整灌胃量。12周之后测定各组大鼠股骨密度和骨钙含量并进行骨组织病理学检查。结果:淫羊藿骨碎补提取物中的主要功效成分为淫羊藿苷和黄酮类物质，含量分别为0.78%和4.38%。12周之后，与模型组相比，中药组、营养剂组的骨密度和骨钙含量均无显著性差异（P>0.05），复方组骨密度和骨钙含量均显著高于模型组（P<0.05）。中药组、营养剂组具有减轻骨质疏松的趋势，但差异不显著（P>0.05），复方组可以增加骨小梁成分，减轻骨小梁稀疏，变细、断裂的程度（P<0.01）。结论:复方组具有增加大鼠骨密度和骨钙含量的作用，可以减轻骨质疏松的程度，增加骨小梁成分，且复方组比单用中药或营养剂效果明显。

Effects of Epimedium Rhizoma Drynariae Combined with Bone Nutritional Tonic on Rats with Osteoporosis

Objective: To study the effects of Epimedium Rhizoma Drynariae deer bone combined with bone nutritional tonic on osteoporosis rats induced by ovariectomized. Methods: Ovariectomized rats were prepared and randomly divided into model group, positive drug group (1.0 mg/kg·bw Estradiol valerate tablets), traditional Chinese medicine group (0.17 g/kg·bw Epimedium Rhizoma Drynariae extract, 0.08 g/kg·bw Deer bone meal), nutritional supplement group (0.17 g/kg·bw Glucosamine hydrochloride, 0.08 g/kg·bw Chondroitin sulfate, 0.17 g/kg·bw Calcium carbonate), compound group (0.17 g/kg·bw Epimedium Rhizoma Drynariae extract, 0.08 g/kg·bw Deer bone meal, 0.17 g/kg·bw Glucosamine hydrochloride, 0.08 g/kg·bw Chondroitin sulfate, 0.17 g/kg·bw Calcium carbonate)and sham operation group. The gavage volume of the above groups was 10 mL/kg·bw, 1 time/day, for 12 successive weeks, adjusted the gavage volume according to the weekly weight. After 12 weeks, the femoral bone density and bone calcium content of rats were measured, and the bone histopathological examination was performed. Results: The main functional components in the hydrating extract of Epimedium Rhizoma Drynariae were icariin (0.78%) and total flavonoids(4.38%). Twelve weeks later, compared with the model group, the bone mineral density and bone calcium content of SD rats in the traditional Chinese medicine group and the nutritional supplement group did not change significantly (P>0.05). The bone density and bone calcium content of SD rats in the compound group were significantly higher than those of rats in the model group (P<0.05). The traditional Chinese medicine group and the nutrient group had a trend to relieve osteoporosis, but it was not significant (P>0.05). The compound could increase the bone trabecular components and reduce the sparseness, thinning and fracture of the bone trabecula (P<0.01). Conclusion: The compound group has the effect of increasing the bone density and bone calcium content of rats, effectively reducing the degree of osteoporosis, and increasing the bone trabecular components. The compound group is more effective than the traditional Chinese medicine or nutrient alone.