| 高效合成L-高丝氨酸大肠杆菌基因工程菌株的构建 |
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| 引用本文： | 张宇,夏利,林蓓蓓,张稳杰,徐皓然,张成林.高效合成L-高丝氨酸大肠杆菌基因工程菌株的构建[J].食品科学,2022,43(6):81-88. |
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| 作者姓名： | 张宇 夏利 林蓓蓓 张稳杰 徐皓然 张成林 |
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| 作者单位： | （天津科技大学生物工程学院，天津 300457） |
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| 基金项目： | 国家自然科学基金青年科学基金项目（31300069）；中国博士后科学基金项目（2017M61170；2018T110662） |
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| 摘 要： | 针对现有L-高丝氨酸生产菌株因营养缺陷需在发酵过程中添加L-苏氨酸等物质的问题,以大肠杆菌为底盘细胞,构建1株非营养缺陷型L-高丝氨酸高产菌株.首先通过下调thrB和thrC的转录弱化L-高丝氨酸的降解途径;并过表达thrA、ppc和pntAB以强化L-高丝氨酸合成代谢流、提高前体物以及辅酶供应;在此基础上过表达rht...
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| 关 键 词： | L-高丝氨酸 代谢工程 大肠杆菌 外排 |
Construction of Escherichia coli Chassis for Efficient Production of L-Homoserine |
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| ZHANG Yu,XIA Li,LIN Beibei,ZHANG Wenjie,XU Haoran,ZHANG Chenglin.Construction of Escherichia coli Chassis for Efficient Production of L-Homoserine[J].Food Science,2022,43(6):81-88. |
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| Authors: | ZHANG Yu XIA Li LIN Beibei ZHANG Wenjie XU Haoran ZHANG Chenglin |
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| Affiliation: | (College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China) |
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| Abstract: | Nutrients such as L-threonine need to be added during the fermentation of L-homoserine by auxotrophic strains. In this study, a non-auxotrophic Escherichia coli chassis for the high-yield production of L-homoserine was constructed. The expression of thrB and thrC were down-regulated to attenuate the degradation of L-homoserine; then the overexpression of thrA, ppc and pntAB was performed to reinforce the synthetic flux and improve precursor supply and NADPH generation; finally, rhtA was overexpressed to elevate L-homoserine efflux. After 48 h fermentation in a 5 L fermenter, the constructed strain H-10 produced 52.1 g/L of L-homoserine with a percentage transformation of glucose of 43%, which is higher than the values reported in the literature. |
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| Keywords: | L-homoserine metabolic engineering Escherichia coli efflux |
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