蜂胶乙醇提取物对小鼠主动脉内皮细胞损伤的保护作用

目的:探究蜂胶乙醇提取物（ethanol extracts of propolis，EEP）对脂多糖（lipopolysaccharide，LPS）诱导的小鼠主动脉内皮细胞（mouse aortic endothelial cell，MAEC）炎症因子损伤的保护作用。方法:将细胞分为对照组，LPS模型组，蜂胶低（2.5 μg/mL）、中（5 μg/mL）、高（10 μg/mL）剂量组。采用CCK-8检测MAEC的细胞增殖率，ELISA酶联免疫吸附实验测定MAEC炎症细胞中TNF-α、IL-6的含量，Western Blot法测定MAEC炎症细胞中ICAM-1、VCAM-1、MCP-1的表达水平。结果:与对照组相比，LPS组MAEC的细胞增殖率极其显著降低（P<0.001），ICAM-1、VCAM-1、MCP-1、TNF-α以及IL-6的水平极其显著升高（P<0.001）。经不同浓度EEP处理后，MAEC的细胞增殖率显著上升（P<0.05或P<0.01），TNF-α、IL-6的含量以及ICAM-1、VCAM-1、MCP-1表达水平降低，各蜂胶组与LPS组相比均有显著性差异（P<0.01或P<0.001）。结论:EEP能够抑制LPS诱导的MAEC中炎症因子的表达，对血管内皮细胞具有保护作用。

Protective Effects of Propolis Ethanol Extract on Mouse Aortic Endothelial Cells Injury

Objective: The aim of this study was to investigate the protective effects of ethanol extracts of propolis (EEP) on mouse aortic endothelial cells (MAEC) from injury induced by lipopolysaccharide (LPS). Methods: Cell proliferation rate was determined by CCK-8. Then MAEC were divided into the blank group, LPS model group, low-dose (2.5 μg/mL), middle-dose (5 μg/mL), and high-dose (10 μg/mL) groups of EEP. The TNF-α and IL-6 levels were evaluated by ELISA. The expression levels of ICAM-1, VCAM-1 and MCP-1 were measured by Western Blot. Results: Compared with the control group, the cell proliferation rate of MAEC in the LPS group was extremely significant reduced (P<0.001), and the levels of ICAM-1, VCAM-1, MCP-1, TNF-α and IL-6 were extremely significant increased (P<0.001). The cell proliferation rate of EEP groups increased significantly (P<0.05 or P<0.01), and the content of TNF-α, IL-6 and the expression levels of ICAM-1, VCAM-1, and MCP-1 decreased compared with these of LPS group. There were significant differences between each propolis group and LPS group (P<0.01 or P<0.001). Conclusion: EEP could effectively inhibit LPS-induced the expression of inflammatory factors in MAEC, and it has the effect of protecting endothelial cells.