UPLC-MS/MS法同时测定采后莲雾果实中7 种酚酸类物质含量

建立一种超高效液相色谱-串联质谱法同时测定莲雾果实中7 种酚酸类物质含量。采用CORTECD? T3色谱柱（2.1 mm×100 mm，2.7 μm），柱温30 ℃。流动相为流速0.3 mL/min的0.1%甲酸-乙腈。样品用乙腈提取2 次，外标法定量。结果表明：7 种酚酸类物质在5.0～1 000 ng/mL范围呈良好线性关系，相关系数r不小于0.996 1，加标回收率为93.6%～98.8%，相对标准偏差为1.9%～7.5%，检出限为0.3～2.7 μg/kg，定量限为1.0～5.0 μg/kg。使用该方法对采后莲雾果实中酚酸类物质含量进行测定，经主成分分析获得了3 个主成分和相应数学评价模型。综合得分F值在贮藏第6天出现负值，说明其酚酸类物质已经开始大量分解。该方法前处理简单，灵敏度高，稳定可靠，可为采后莲雾果实品质研究提供参考。

Simultaneous Determination of Seven Phenolic Acids in Wax Apple Fruit by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

An ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established for the analysis of seven phenolic acids in wax apple fruit. The separation was performed on a Waters CORTECD? T3 (2.1 mm × 100 mm, 2.7 μm) column, and the column temperature was set at 30 ℃. The mobile phase consisted of 0.1% formic acid aqueous solution and acetonitrile at a 0.3 mL/min flow rate. The samples were extracted twice with acetonitrile, and the external standard method was used for quantitative detection of the phenolic acids. Results showed that the standard calibration curves had a good linear relationship in the range of 5.0–1 000 ng/mL with correlation coefficients equal to or larger than 0.996 1. The recoveries of spiked samples ranged from 93.6% to 98.8%, with relative standard deviation (RSD) from 1.9% to 7.5%. The limits of detection were 0.3–2.7 μg/kg, and the limits of quantification were 1.0–5.0 μg/kg. This method was used to determine phenolic acids in wax apple fruit, and the obtained data were analyzed by principal component analysis (PCA). Three principal components were obtained and used as the independent variables for proposing a mathematical model. The comprehensive F value was negative on the 6th day of storage, indicating that a large number of phenolic acids had begun to decompose. In conclusion, the established method is simple, sensitive, stable, reliable, and suitable for the quality evaluation of wax apple.