高效液相色谱-三重四极杆串联质谱法研究塑料接触材料中酚类化合物向奶油中的迁移

建立奶油中14 种酚类化合物高效液相色谱-三重四极杆串联质谱的同时测定方法，研究裱花蛋糕塑料接触材料中酚类化合物在真实样品奶油中的迁移情况。塑料样品经奶油浸泡，乙腈-甲醇提取，T3色谱柱分离，以负离子-多反应监测模式进行测定，基质外标法定量；分析双酚A（bisphenol A，BPA）和4-壬基酚（4-nonylphenol，4-NP）在奶油样品中的迁移规律，用于14 种酚类化合物在奶油中迁移量的测定研究。结果表明，14 种酚类化合物的检出限为0.15～0.75 μg/kg，定量限为0.5～2.5 μg/kg，线性关系良好，相关系数均大于0.99；酚类物质在动物和植物奶油中的加标回收率分别为78.2%～117.1%、79.4%～114.6%，相对标准偏差分别为1.07%～12.1%、1.12%～12.5%；塑料接触材料中BPA和4-NP在奶油中的迁移量随着温度的升高和时间的延长逐渐增大。采用本方法对市售塑料蛋糕装饰摆件等在动物奶油中的迁移量等进行测定，迁出化合物BPA和双酚S迁移量最高值分别为4.61、0.94 μg/kg；4-NP迁移量最高值为125.1 μg/kg，检出样品数量最多。酚类化合物迁移到食品中的风险性应引起关注。

Migration of Phenolic Compounds from Plastic Contact Materials into Cream by High Performance Liquid Chromatography-Tandem Mass Spectrometry

An analytical method was established for the determination of 14 phenolic compounds in cream by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), and the migration of phenolic compounds from plastic contact materials into cream was studied. The samples were soaked in cream, extracted with acetonitrile/methanol, and separated on a T3 column. The analytes were detected in negative electrospray ionization (ESI) mode with multiple reaction monitoring (MRM), and quantified by the matrix-matched external standard method. The migration of bisphenol A (BPA) and 4-nonylphenol (4-NP) from plastic contact materials into cream was analyzed. The results showed that the limits of detection of the 14 phenolic compounds were 0.15–0.75 μg/kg and the limits of quantification were 0.5–2.5 μg/kg. A good linear relationship was observed for all analytes with correlation coefficients greater than 0.99. The recoveries for spiked whipping cream and non-dairy cream were 78.2%–117.1% and 79.4%–114.6% with relative standard deviations (RSDs) of 1.07%–12.1% and 1.12%–12.5%, respectively. The migration of BPA and 4-NP into cream increased with increasing temperature and contact time. The highest levels of migration of BPA, BPS and 4-NP from plastic cake decorations into cream w were determined to be 4.61, 0.94 and 125.1 μg/kg, respectively. The number of samples that tested positive for 4-NP was the largest. Much attention should be paid to the risk of migration of phenolic compounds into foods.