采后水杨酸处理对金刺梨果实活性氧和苯丙烷代谢的影响

以金刺梨为材料，采后采用1.5 mmol/L水杨酸溶液浸泡10 min处理，并对果实贮藏期间腐烂率、失重率、细胞膜完整性、活性氧代谢以及苯丙烷代谢关键酶与代谢产物等指标进行测定，研究水杨酸对金刺梨果实活性氧代谢和苯丙烷代谢的影响。结果表明: 1.5 mmol/L水杨酸溶液浸泡处理可有效降低贮藏期间果实的失重率、腐烂率以及保持果实细胞膜完整性。提高了果实中O₂?·产生速率以及H₂O₂含量，同时提高了超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）的活性。另外，处理还提高了果实苯丙氨酸解氨酶（PAL）和4-香豆酰-辅酶A连接酶（4CL）活性，诱导果实中总酚和木质素的积累。由此表明，水杨酸可通过激活金刺梨果实体内抗氧化酶活性，苯丙烷代谢相关酶活性以及抗菌物质的积累来提高果实抗氧化能力，延缓果实衰老。

Effects of Salicylic Acid Treatment on Reactive Oxygen Species Metabolism and Phenylpropanoid Pathway in Rosa sterilis

The Rosa sterilis were used as the material, which were treated with 1.5 mmol/L salicylic acid solutions for 10 min. The change of decay rate, weight loss rate, cell membrane integrity and the key enzymes and metabolites of reactive oxygen species metabolism and phenylpropanoid pathway were analyzed to reveal its effects on the reactive oxygen species metabolism and phenylpropanoid pathway. The result showed that the 1.5 mmol/L salicylic acid solution soaking treatment effectively reduced the rate of weight loss and decay, and maintained the cell membrane integrity during fruit storage time. Salicyli cacid significantly increased O₂?· production rate, H₂O₂ content and the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in Rosa sterilis. In addition, salicylic acid also increased the activities of phenylalanine ammonia lyase (PAL) and 4-coumarin-CoA ligase (4CL), and induced the accumulation of total phenolic compounds and lignin in the fruits. These results suggested that salicylic acid can enhance the antioxidant capacity and delay senescence in Rosa sterilis by activating the antioxidant enzyme activity, key enzyme activities of phenylpropanoid pathway and accumulation of antibacterial substances.