响应面法优化托盘根总皂苷提取工艺及抗氧化活性研究

为进一步开发托盘根的功能性成分和药用价值.本研究以托盘的干燥根为试材,通过考察提取时间、乙醇体积分数、液料比等因素对托盘根总皂苷得率的影响,选用响应面法(RSM)探究托盘根总皂苷的最佳提取工艺,且研究了托盘根总皂苷提取物的体外抗氧化能力.结果表明:托盘根总皂苷的最优提取条件为:提取时间2 h,乙醇体积分数71％,液料比...

Optimization of Extraction Process of Total Saponins from Rubus crataegifolius Bunge. Root by Response Surface Methodology and Its Antioxidant Activity

In this study, the extraction process of total saponins from Rubus crataegifolius Bunge. roots was investigated and optimized by response surface method. The effects of ethanol concentration, extraction time and solid-liquid ratio on the extraction rate of total saponins from pallets were investigated. The results showed that the optimal extraction conditions of the total saponins from the pallets were: extraction time 2 h, ethanol concentration 71%, liquid-solid ratio 28:1 mL/g, the predicted value was 3.49%. Under these conditions, the extraction rate of total saponins from R. crataegiolius Bunge. roots was 3.366%. The IC₅₀ (mg/mL) values of total saponins extract from R. crataegifolius Bunge. roots against DPPH·, ABTS+· and superoxide anion free radicals were 0.0782, 0.277 and 0.9954 mg/mL, respectively. Therefore, the response surface method to optimize the extraction process of total saponins from R. crataegifolius Bunge. roots was effective, reliable, stable and feasible, and this article would provide scientific basis for the development and utilization of total saponins from pallets as natural antioxidants.