气相色谱-质谱联用法测定茶叶包装纸中9, 10-蒽醌含量

建立气相色谱-质谱联用（GC-MS-MS）检测茶叶包装纸中9, 10-蒽醌方法。样品采用丙酮-正己烷混合溶液提取，提取液经离心、氮吹浓缩后，过弗罗里硅土固相萃取柱SPE净化，净化液进入气质联用仪分析，仪器采用DB-5MS气相色谱柱（20 m×0.18 mm×0.18 μm）分离，选择多反应监测模式扫描，内标法定量。结果表明，该方法在10~200 ng/mL质量浓度范围内具有良好的线性关系，线性回归方程为Y=0.23249X+0.001203，拟合度R2为0.9989，方法检出限为3 μg/kg，定量限为10 μg/kg，加标回收率在89.3%~98.7%之间，相对标准偏差（RSD）为3.01%~4.42%。该方法适用于茶叶包装纸中9，10-蒽醌定性定量检测。

Determination of 9, 10-Anthraquinone in Tea Packaging Paper by GC-MS/MS

A gas chromatography-mass spectrometry (GC-MS-MS) method was established for the detection of 9, 10-anthraquinone in tea packaging paper. The sample was extracted with acetone-n-hexane mixed solution, and then the extract was concentrated by centrifugation and nitrogen blowing, and then purified by SPE with a Florisil solid phase extraction column. The purified liquid was analyzed by a gas-mass spectrometer. The instrument used DB-5MS gas chromatography. Column (20 m×0.18 mm×0.18 μm) was separated, scanning in multi-reaction monitoring mode was selected, and internal standard method was used for quantification. The results showed that the method had a good linear relationship in the mass concentration range of 10~200 ng/mL, the linear regression equation was Y=0.23249X+0.001203, the fit R2 was 0.9989, the detection limit of the method was 3 μg/kg, the limit of quantification was 10 μg/kg, the recovery rate of standard addition was 89.3%~98.7%, and the relative standard deviation (RSD) was 3.01%~4.42%. This method was suitable for the qualitative and quantitative detection of 9, 10-anthraquinone in tea packaging paper.