HPLC法测定乳制品中二氢槲皮素含量

本研究旨在建立高效液相色谱法测定乳制品中新型食品添加剂二氢槲皮素含量。二氢槲皮素经乙腈/水溶液提取，含亲水基团的二乙烯基苯/聚苯乙烯共聚物萃取小柱（HLB或PLS）净化，C₁₈反向色谱柱分离后紫外检测器进行检测。方法学结果表明，二氢槲皮素线性范围为0.5~20.0 μg/mL，线性相关系数为0.9997，不同类别样品回收率范围为86.0%~101.0%，精密度RSD为2.06%（n=6）。本方法重现性好，准确度高，可以为食品中二氢槲皮素的检测研究及国家标准的建立提供参考依据。

Determination of Dihydroquercetin in Dairy Products by HPLC

The aim of this study was to establish a method for the determination of dihydroquercetin, a new food additive, in dairy products by High-performance liquid chromatography. The dihydroquercetin was extracted by acetonitrile /water solution, then was purified by extraction column with Polystyrene / divinylbenzene Copolymer (HLB or PLS). After separation by C₁₈ reversed-phase chromatographic column, the content of dihydroquercetin was detected by the UV detector detection. The results showed that the linear range of dihydroquercetin was 0.5~20 μg/mL, the linear correlation coefficient was 0.9997, the recovery range of different kinds of samples was 86.0%~101%, the precision RSD was 2.06% (n=6).The method has good reproducibility and high accuracy. It can provide reference for the determination of dihydroquercetin in food and the establishment of national standard.