| 植物乳杆菌BLPC002产苯乳酸发酵工艺优化 |
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| 引用本文： | 黄国昌,顾斌涛,于一尊,熊大维,章帅文. 植物乳杆菌BLPC002产苯乳酸发酵工艺优化[J]. 中国酿造, 2021, 40(12): 75. DOI: 10.11882/j.issn.0254-5071.2021.12.014 |
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| 作者姓名： | 黄国昌 顾斌涛 于一尊 熊大维 章帅文 |
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| 作者单位： | （1.江西省科学院微生物研究所，江西 南昌 330096；2.江西省科学院，江西 南昌 330096） |
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| 基金项目： | 江西省重点研发计划（20192BBFL60032）；江西省重点研发计划（20202BBF63014） |
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| 摘 要： | 为提高植物乳杆菌（Lactobacillus plantarum）BLPC002发酵产苯乳酸，通过单因素试验和Box-Behnken试验设计对菌株BLPC002发酵产苯乳酸的工艺条件进行优化。结果表明，菌株BLPC002产苯乳酸最佳发酵工艺条件为：摇床接种量2.5%、转速200 r/min、装液量170 mL/500 mL和发酵温度33 ℃。在该优化条件下，苯乳酸产量为2.66 g/L，是优化前的1.6倍。
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| 关 键 词： | 植物乳杆菌 苯乳酸 发酵条件优化 响应面法 |
Optimization of fermentation process of phenyllactic acid production by Lactobacillus plantarum BLPC002 |
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| HUANG Guochang,GU Bintao,YU Yizun,XIONG Dawei,ZHANG Shuaiwen. Optimization of fermentation process of phenyllactic acid production by Lactobacillus plantarum BLPC002[J]. China Brewing, 2021, 40(12): 75. DOI: 10.11882/j.issn.0254-5071.2021.12.014 |
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| Authors: | HUANG Guochang GU Bintao YU Yizun XIONG Dawei ZHANG Shuaiwen |
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| Affiliation: | (1.Institute of Microbiology, Jiangxi Academy of Sciences, Nanchang 330096, China; 2.Jiangxi Academy of Sciences, Nanchang 330096, China) |
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| Abstract: | In order to improve the yield of phenyllactic acid by fermentation of Lactobacillus plantarum BLPC002, the process conditions for phenyllactic acid production by strain BLPC002 were optimized by single factor experiments and Box-Behnken design experiments. The results showed that the optimal fermentation conditions for phenyllactic acid production by strain BLPC002 were as follows: inoculum 2.5%, rotating speed 200 r/min, liquid volume 170 ml/500 ml and fermentation temperature 33 ℃. Under the optimized conditions, the yield of phenyllactic acid was 2.66 g/L, which was 1.6 times that of before optimization. |
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| Keywords: | Lactobacillus plantarum phenyllactic acid fermentation conditions optimization response surface method |
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