柱切换法液相色谱测定保健食品中的VA、VD、VE

建立柱切换法液相色谱测定保健食品中VA、VD、VE含量的方法.参照GB 5009.82-2016《食品中维生素A、D、E的测定》样品前处理方法,以Agilent Poroshell 120 PFP(4.6 mm×100 mm,2.7 μm)为一维色谱柱,甲醇、水为流动相梯度洗脱,分离VA和VE的4种异构体,以Agile...

Determination of Vitamins A,D and E in Health Foods Using Column-Switching Liquid Chromatography

A method for the determination of vitamins A (VA), D (VD) and E (VE) in health foods by column-switching two-dimensional liquid chromatography was established. According to the sample pretreatment method specified in the national standard GB 5009.82-2016, VA and four isomers of VE were separated on an Agilent Poroshell 120 PFP column (4.6 mm × 100 mm, 2.7 μm) as one-dimensional chromatographic column using a mobile phase consisting of methanol and water by gradient elution, and VD2 and VD3 were separated on an Agilent Zorbax Eclipse PAH column (2.1 mm × 100 mm, 3.5 μm) by gradient elution using a mobile phase composed of methanol and acetonitrile. VD was captured on an Agilent Poroshell 120 EC C18 column (4.6 mm × 5 mm, 4 μm). According to the peak time of VD on the one-dimensional chromatographic column, the column switching time was determined. The one-dimensional detection wavelengths were 325 and 294 nm, and the two-dimensional detection wavelength was 264 nm. The analytes were quantified by external standard method. Results indicated that the calibration curves were linear in the range of 2.50-50.0 μg/mL for VA, and 0.10-2.00 μg/mL for VD, and 5.00-100.0 μg/mL for VE, and the correlation coefficients were all more than 0.999. The recoveries were 86%–102%, with relative standard deviations (RSD) of 1.5%–4.0%. The results suggest that the developed method is accurate and suitable for the determination of VA, VD and VE in health foods.