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人参叶中高产皂苷菌种的筛选及培养条件优化
引用本文:冯颖,陶亮,肖学爱,赵存朝,李畅,田洋.人参叶中高产皂苷菌种的筛选及培养条件优化[J].中国酿造,2021,40(8):117.
作者姓名:冯颖  陶亮  肖学爱  赵存朝  李畅  田洋
作者单位:(1.云南农业大学 食品科学技术学院,云南 昆明 650201;2.食药同源资源开发与利用教育部工程研究中心,云南 昆明 650201; 3.国家辣木加工技术中心,云南 昆明 650201)
基金项目:云南省中青年学术和技术带头人后备人才(2018HB040);云南绿色食品国际合作研究中心项目(2019ZG00905);重大专项(202002AA100005)
摘    要:采用微生物发酵提取吉林不同产地(长白山、通化市榆林镇及白山市抚松县)人参叶皂苷,筛选适合发酵人参叶的最佳菌种,并研究料液比、发酵温度、接种量、初始pH值等因素对皂苷含量的影响,同时研究添加外源氮源、碳源、有机酸及磷盐等对发酵皂苷释放量的影响,确定最佳发酵条件及培养基成分。结果表明,最适的发酵菌种为酿酒酵母(Saccharomyces cerevisiae)CICC 31393,最佳发酵条件为料液比1∶10(g∶mL)、发酵温度30 ℃、接种量2.0%、初始pH值为5.0;最佳发酵培养基为蛋白胨+酵母粉(1∶1)1%、果糖2%、丙酮酸0.14%、磷酸氢二钾0.08%。在此优化条件下,3个不同产地人参叶发酵后总皂苷产量分别为194.25 mg/g、214.57 mg/g、271.75 mg/g。较未发酵组产量(64.11 mg/g、86.19 mg/g、106.91 mg/g)平均提高了约2.5倍。

关 键 词:人参叶  菌株筛选  培养条件优化  人参皂苷  

Screening of high-yield ginsenoside strains from ginseng leaves and culture conditions optimization
FENG Ying,TAO Liang,XIAO Xueai,ZHAO Cunchao,LI Chang,TIAN Yang.Screening of high-yield ginsenoside strains from ginseng leaves and culture conditions optimization[J].China Brewing,2021,40(8):117.
Authors:FENG Ying  TAO Liang  XIAO Xueai  ZHAO Cunchao  LI Chang  TIAN Yang
Affiliation:(1.College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China; 2.Development and Utilization of Food and Drug Homologous Resources Engineering Research Center, Ministry of Education, Kunming 650201, China; 3.National Moringa Processing Technology Center, Kunming 650201, China)
Abstract:The ginsenosides were extracted from ginseng leaves from different producing areas in Jilin (Changbai mountain, Yulin town in Tonghua, Fusong county in Baishan) by microbial fermentation, the optimal strains suitable for fermenting ginseng leaves were screened, and the effects of fermentation conditions such as material to liquid ratio, fermentation temperature, inoculum, and initial pH on the content of ginsenosides were studied. Meanwhile, the effects of the addition of exogenous nitrogen sources, carbon source, organic acids and phosphate salts on the release of ginsenosides were studied, and the optimal fermentation conditions and medium components were determined. The results showed that the optimal fermentation strain was Saccharomyces cerevisiae CICC 31393, the optimal fermentation conditions were material to liquid ratio 1∶10 (g∶ml), fermentation temperature 30 ℃, inoculum 2.0% and initial pH value 5.0, and the optimal fermentation medium was peptone and yeast powder (1∶1) 1%, fructose 2%, pyruvic acid 0.14%, and dipotassium phosphate 0.08%. Under these optimized conditions, the yield of the total ginsenosides from ginseng leaves from three different producing areas after fermentation were 194.25 mg/g, 214.57 mg/g and 271.75 mg/g, respectively. Compared with the unfermented group (64.11 mg/g, 86.19 mg/g, 106.91 mg/g), the yields increased by about 2.5 times on average.
Keywords:ginseng leaf  strain screening  culture conditions optimization  ginsenoside  
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