| 具有保护肠上皮细胞HT-29免受大肠杆菌和H2O2损伤潜力的植物乳杆菌的筛选 |
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| 引用本文： | 马长路,柳青,李淑荣,张丽娟,妥彦峰. 具有保护肠上皮细胞HT-29免受大肠杆菌和H2O2损伤潜力的植物乳杆菌的筛选[J]. 食品科学, 2022, 43(5): 143-150. DOI: 10.7506/spkx1002-6630-20210421-299 |
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| 作者姓名： | 马长路 柳青 李淑荣 张丽娟 妥彦峰 |
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| 作者单位： | （1.北京农业职业学院食品与生物工程系，北京 102442；2.大连工业大学食品学院，辽宁 大连 116034） |
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| 基金项目： | 北京市农业科技项目（20200124）；北京农业职业学院科技援助项目（XY-BZ-20-01） |
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| 摘 要： | 益生菌可在肠道定植从而发挥抗炎或抗氧化活性,有利于宿主肠道健康.本实验研究了从新疆传统发酵乳制品中分离得到的8株植物乳杆菌对大肠杆菌侵袭和过氧化氢刺激肠上皮细胞HT-29的保护作用.结果表明:在8株植物乳杆菌中,植物乳杆菌35具有最高的黏附能力.植物乳杆菌35可通过取代、竞争、排阻的方式抑制大肠杆菌对HT-29细胞的黏...
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| 关 键 词： | 乳杆菌 抑制黏附 大肠杆菌O157 人结肠癌细胞HT-29 |
Screening Lactobacillus plantarum Strains with Potential to Protect Human Intestinal Epithelium HT-29 Cells against Injury Caused by Escherichia coli or H2O2 |
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| MA Changlu,LIU Qing,LI Shurong,ZHANG Lijuan,TUO Yanfeng. Screening Lactobacillus plantarum Strains with Potential to Protect Human Intestinal Epithelium HT-29 Cells against Injury Caused by Escherichia coli or H2O2[J]. Food Science, 2022, 43(5): 143-150. DOI: 10.7506/spkx1002-6630-20210421-299 |
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| Authors: | MA Changlu LIU Qing LI Shurong ZHANG Lijuan TUO Yanfeng |
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| Affiliation: | (1. Department of Food and Biological Engineering, Beijing Vocational College of Agriculture, Beijing 102442, China; 2. School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China) |
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| Abstract: | Probiotic Lactobacillus strains can exert anti-inflammatory or antioxidant activities after colonization in the gut, which is beneficial for the host’s intestinal health. In this study, eight L. plantarum strains isolated from traditional fermented dairy foods in Xinjiang, China, were evaluated for their protective effects on HT-29 human intestinal epithelial cells against injury caused by Escherichia coli or hydrogen peroxide (H2O2). Among these strains, L. plantarum 35 showed the highest adhesion capacity to HT-29 cell monolayers, and it reduced E. coli adhesion to HT-29 cells by displacement, competition, and exclusion, with inhibition rates of 42.60%, 59.17%, and 60.19%, respectively. L. plantarum 35 and its crude exopolysaccharide (EPS) inhibited E. coli from stimulating HT-29 cells to produce interleukin-8 (IL-8), protected HT-29 cells against injury induced by H2O2, increased the levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and decreased malondialdehyde (MDA) content in HT-29 cells. Therefore, L. plantarum 35 and its crude EPS have the potential to inhibit inflammatory bowel disease induced by E. coli O157. |
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| Keywords: | Lactobacillus anti-adhesion Escherichia coli O157 HT-29 cells, |
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