响应面法优化铁皮石斛多糖纯化工艺

目的:用响应面法优化铁皮石斛粗多糖的双水相萃取纯化工艺.方法:通过单因素实验考察了(NH4)2SO4质量分数、C2H5OH质量分数和萃取温度对多糖萃取率和除蛋白率的影响,进而进行响应面试验,以多糖萃取率和除蛋白率为响应值进行统计分析.结果:各因素对多糖萃取率和除蛋白率的影响程度由大到小依次为萃取温度＞(NH4)2SO4...

Optimization of Purification Process of Polysaccharides from Dendrobium officinale by Response Surface Methodology

Objective: To optimize the purification process of crude polysaccharides from Dendrobium officinale by using response surface methodology. Methods: Through single factor experiments, the effects of (NH₄)₂SO₄ concentration, C₂H₅OH concentration and extraction temperature on the extraction rate and protein removal rate of polysaccharides were investigated, and then a response surface test was performed. The response values of the extraction rate and protein removal rate of polysaccharides were used for statistical analysis. Results: The degree of influence of various factors on the polysaccharides extraction rate and protein removal rate in descending order was extraction temperature > (NH₄)₂SO₄ concentration > C₂H₅OH concentration. Based on the total mass of the two-phase aqueous system, the optimal extraction conditions were as follows: 18.80% (NH₄)₂SO₄, 25.00% C₂H₅OH, extraction temperature was 25 ℃, and the Dendrobium officinale polysaccharide extraction rate and protein removal rate were respectively 94.39% and 91.36%. Conclusion: This method could effectively purify the crude polysaccharides of Dendrobium officinale, and provided a theoretical basis for the development and utilization of Dendrobium officinale polysaccharides.