紫苏籽多糖分离纯化及抗肿瘤活性

为了探究紫苏籽多糖（Perilla frutescens seed polysaccharide,PFSP）的抗肿瘤活性，利用传统方法从紫苏籽中提取粗多糖，利用DEAE-52纤维素和葡聚糖G-100柱层析分离纯化获得紫苏籽多糖，并测定该多糖对H22荷瘤小鼠免疫系统的影响。结果表明，紫苏籽粗多糖得率为8.38%，分离纯化后得到3个组分，分别命名为PFSP-1、PFSP-2和PFSP-3。PFSP-1由甘露糖、半乳糖、木糖及阿拉伯糖（物质的量之比为0.01∶0.06∶0.11∶0.81）组成，PFSP-2由甘露糖、木糖及阿拉伯糖（物质的量之比为0.28∶0.28∶0.41）组成，PFSP-3由鼠李糖、葡萄糖醛酸、葡萄糖、半乳糖、木糖及阿拉伯糖（物质的量之比为0.013∶0.024∶0.040∶0.080∶0.120∶0.700）组成；3个组分均具有多糖特征吸收峰，为吡喃环型多聚糖。抗肿瘤实验表明，PFSP-2抑瘤率显著高于PFSP-1和PFSP-3(P<0.05)；对PFSP-2进一步研究发现，与模型组相比，PFSP-2可显著降低乳酸脱氢酶、醛缩酶和白细胞介素（interleukin...

Separation,Purification and Anti-tumor Activity of Polysaccharides from Perilla frutescens Seeds

In order to explore the anti-tumor activity of Perilla frutescens seed polysaccharide (PFSP), the crude polysaccharide was obtained from Perilla frutescens seeds by traditional solvent extraction and purified by DEAE-52 cellulose and dextran G-100 column chromatography, and the effect of the purified polysaccharide on the immune system of H22 tumor bearing mice was determined. The results showed that the yield of the crude polysaccharide was 8.38%. After separation and purification, three polysaccharide fractions, named PFSP-1, PFSP-2 and PFSP-3, were obtained. PFSP-1 was composed of mannose, galactose, xylose and arabinose (at a molar ratio of 0.01:0.06:0.11:0.81). PFSP-2 was composed of mannose, xylose and arabinose (at a molar ratio of 0.28:0.28:0.41). PFSP-3 was composed of rhamnose, glucuronic acid, glucose, galactose, xylose and arabinose (at a molar ratio of 0.013:0.024:0.040:0.080:0.120:0.700). The three polysaccharides all displayed absorption peaks characteristic of polysaccharides and were identified as pyran-type polysaccharides. The anti-tumor effect of PFSP-2 was significantly higher than that of PFSP-1 and PFSP-3 (P < 0.05). Furthermore, PFSP-2 could significantly reduce the concentrations of lactate dehydrogenase and aldolase and interleukin (IL)-10 (P < 0.05), increase the concentrations of IL-2 and tumor necrosis factor α, down-regulate the expression of antiapoptotic protein Bcl-2, and up-regulate the expression of apoptotic protein Bax compared with the model group (P < 0.05). These results indicate that PFSP-2 can inhibit the growth of tumor cells in vivo by improving the immune system of mice.