基于高效液相色谱-质谱联用技术检测畜肉中的瓜尔胶

建立畜肉中瓜尔胶的高效液相色谱-质谱测定法。样品经β-甘露聚糖酶酶解，加入乙腈沉淀蛋白后离心，上清液经过中性氧化铝柱净化，用高效液相色谱-串联质谱仪测定，外标法定量。结果表明，瓜尔胶在线性范围为0～2 g/kg，线性相关系数在0.99以上，检出限为0.030 0 g/kg，定量限为0.100 g/kg，回收率为85.4%～109.8%，相对标准偏差在1.5%～9.3%之间。该方法前处理简便、检测指标明确、灵敏度高，可作为定量检测畜肉中添加瓜尔胶的有效手段。

Detection of Guar Gum in Adulterated Livestock Meat by High Performance Liquid Chromatography-Mass Spectrometry

This study proposed a high performance liquid chromatography-mass spectrometry (HPLC-MS) method for the determination of guar gum in adulterated livestock meat. The samples were enzymatically hydrolyzed by β-mannanase, added with acetonitrile to precipitate protein, and then centrifuged. The supernatant was purified on a neutral alumina column, detected using a liquid chromatography-tandem mass spectrometer, and quantified by external standard method. The results showed that the linear range for guar gum was 0–2 g/kg, the correlation coefficient was above 0.99, the limit of detection (LOD) was 0.030 0 g/kg, the limit of quantification (LOQ) was 0.100 g/kg, and the recoveries ranged from 85.4% to 109.8%, with relative standard deviations (RSDs) between 1.5% and 9.3%. The developed method has the advantages of simple pretreatment, clear detection indexes and high sensitivity, and can be used as an effective method for detecting added guar gum in livestock meat.