基于UPLC-QE-MS探究褐变反应对酸乳中代谢物的影响

采用超高效液相色谱-四极杆静电场轨道阱质谱非靶向代谢组学的方法,探究普通酸乳与褐色酸乳代谢物的差异,并从代谢物的角度评估2种酸乳的不同.在正负离子模式下采集2种酸乳的代谢物信息并结合多元统计分析方法对2种酸乳的代谢物进行筛选,共得到117种差异性代谢物.与普通酸乳相比,褐色酸乳中有106种代谢物的相对含量上调,11种代...

Effect of Browning Reaction on the Metabolite Profile of Yogurt Analyzed by Untargeted Metabolomics Based on Ultra-high Performance Liquid Chromatography-Quadrupole Electrostatic Field Orbitrap Mass Spectrometry

In this study, untargeted metabolomics based on ultra-high performance liquid chromatography-quadrupole electrostatic field orbitrap mass spectrometry (UPLC-QE-MS) was used to explore the differences in metabolite profiles between ordinary yogurt and brown yogurt, and the differences between them were evaluated from the perspective of metabolites. The metabolite information of yogurts was collected in both the positive and negative ion modes, and the metabolites were screened by multivariate statistical analysis. A total of 117 differential metabolites were obtained. Compared with ordinary yogurt, the relative contents of 106 metabolites were up-regulated and 11 metabolites were down-regulated in brown yogurt. Compared with ordinary yogurt, the contents of 3 flavor amino acids, 8 peptides, 12 sugars, 2 Maillard reaction products and one quinone in brown yogurt changed significantly. The results of this study can provide some theoretical support for the future research and development of brown yogurt.