双酶协同降解胶体几丁质及其作用机制

建立一种几丁质酶(chitinase,ChiA)和β-N-乙酰基己糖胺酶HJ5N协同催化体系,可实现一步反应降解胶体几丁质制备N-乙酰氨基葡萄糖(N-acetylglucosamine,GlcNAc),探讨不同因素对双酶协同催化体系的影响和双酶协同作用机制.结果表明,双酶协同催化体系的最适反应温度为30℃,最适反应pH...

Synergistic Enzymatic Degradation of Colloidal Chitin and Its Mechanism

In this study, a synergistic catalytic system of chitinase (ChiA) and β-N-acetylhexosaminase HJ5N that can degrade colloidal chitin to prepare N-acetylglucosamine (GlcNAc) in one-step reaction was established. The effects of different factors on the catalytic efficiency and the mechanism of the synergistic action of the enzymes were discussed. The results showed that the optimum reaction conditions were 30 ℃, pH 6 and concentration ratio ChiA to HJ5N of 2:1. Under these conditions, 17.55 mg/mL of GlcNAc was produced with a yield of 58% when 90 g/L of (containing 30.3 g/L of colloidal chitin) was used as the substrate. HJ5N could ensure quick conversion of the intermediate product (GlcNAc)2 into GlcNAc, thus removing the inhibitory effect of (GlcNAc)2 on ChiA and ultimately allowing the efficient degradation of colloidal chitin into GlcNAc. This study provides an effective strategy for the industrial application of enzymatic degradation of colloidal chitin to prepare N-acetylglucosamine.