| 玉米活性肽的分离纯化及抑制体外脂质过氧化作用研究 |
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| 引用本文： | 陈红漫,李艳秋,阚国仕,杨奉仪,王丽娜.玉米活性肽的分离纯化及抑制体外脂质过氧化作用研究[J].食品科技,2011(9):271-275. |
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| 作者姓名： | 陈红漫 李艳秋 阚国仕 杨奉仪 王丽娜 |
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| 作者单位： | 沈阳农业大学生物科学技术学院;黑龙江省农业科学院大庆分院; |
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| 基金项目： | 辽宁省教育厅A类基金项目(05L394) |
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| 摘 要： | 以玉米醇溶蛋白为底物,利用碱性蛋白酶进行水解反应获得了玉米活性肽组分,经离子交换和葡聚糖凝胶等方法对产物进一步分离纯化。以H2O2及Fe2+诱导的小鼠体外脂质过氧化反应为靶点,研究了玉米活性肽及其分离纯化组分对脂质过氧化的抑制作用。结果表明:活性肽及其分离纯化组分均具有抗脂质过氧化作用,其对小鼠肝组织体外脂质化抑制率达到30.65%。其中组分Ⅰ的抑制活性最强,分子量主要分布在400~1000u。
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| 关 键 词： | 玉米活性肽 分离纯化 脂质过氧化 抗氧化 |
Purification of corn active peptide and its inhibiting lipid peroxidation |
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| CHEN Hong-man,LI Yan-qiu,KAN Guo-shi,YANG Feng-yi,WANG Li-na.Purification of corn active peptide and its inhibiting lipid peroxidation[J].Food Science and Technology,2011(9):271-275. |
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| Authors: | CHEN Hong-man LI Yan-qiu KAN Guo-shi YANG Feng-yi WANG Li-na |
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| Affiliation: | CHEN Hong-man1,LI Yan-qiu1,KAN Guo-shi1,YANG Feng-yi1,WANG Li-na2(1.Collage of Biotechnology,Shenyang Agriculture University,Shenyang 110866,2.Daqing Branch of Heilongjiang Academy of Agricultural Science,Daqing 163319) |
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| Abstract: | Zein was hydrolyzed by proteolysis with alkaline protease. The hydrolysate was further purified by means of ion-exchange and gel filtration chromatography. With H2O2 and Fe2+ as the inducing reagent, the anti-oxidant ability of corn peptide and its separated fraction were examined for the effect of spontaneous lipid peroxidation. The results reveal that corn peptide and its separated fraction exhibited a effect of inhibiting rate of the spontaneous lipid peroxidation in liver tissue was 30.65 % of the norma... |
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| Keywords: | corn active peptide separation and purificationl spontaneous lipid peroxidation antioxidant |
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