| 己烯雌酚残留检测ELISA试剂盒的研制及应用 |
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| 引用本文： | 梁康建,白瑞樱,宋利君,沈玉栋,徐振林,王弘,雷红涛,杨金易.己烯雌酚残留检测ELISA试剂盒的研制及应用[J].食品科技,2011(9):347-352. |
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| 作者姓名： | 梁康建 白瑞樱 宋利君 沈玉栋 徐振林 王弘 雷红涛 杨金易 |
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| 作者单位： | 广东省食品质量安全重点实验室华南农业大学食品质量安全研究所;新乡医学院生理学与神经生物学教研室; |
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| 基金项目： | 国家自然科学基金项目(30901005); 广东省科技计划项目(2009B011300005,2009A020101004); 广东省教育部产学研结合项目(2010A090200084) |
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| 摘 要： | 在实验室前期制备的己烯雌酚多克隆抗体基础上建立间接竞争ELISA检测方法,研制出己烯雌酚残留ELISA快速检测试剂盒,并对其性能参数进行测定。结果表明,所选用的抗体对己烯雌酚亲和力高,除了与双烯雌酚、己烷雌酚的交叉反应率分别为6.83%和2.56%外,与其他结构类似物或功能类似物交叉反应率均小于0.01%;己烯雌酚残留检测的标准曲线为y=-28.71x+59.37,R2为0.9895,线性范围为0.08~8.17ng/mL,检测限IC10为0.05ng/mL,灵敏度IC50为1.09ng/mL,样品的加标回收率为80.04%~107.91%,平均批内和批间变异系数分别为6.41%和8.83%;与HPLC测定方法比对具有较高的相符性,相关系数R2为0.9882;该试剂盒在4℃下至少可保存6个月,可用于批量猪肉、鸡肉、鱼肉、饲料等样品中己烯雌酚残留的检测。
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| 关 键 词： | 己烯雌酚 多克隆抗体 间接竞争ELISA 试剂盒 |
Development and application of ELISA kit for detectingdiethylstilbestrol residue |
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| LIANG Kang-jian,BAI Rui-ying,SONG Li-jun,SHEN Yu-dong,XU Zhen-lin,WANG Hong,LEI Hong-tao,YANG Jin-yi.Development and application of ELISA kit for detectingdiethylstilbestrol residue[J].Food Science and Technology,2011(9):347-352. |
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| Authors: | LIANG Kang-jian BAI Rui-ying SONG Li-jun SHEN Yu-dong XU Zhen-lin WANG Hong LEI Hong-tao YANG Jin-yi |
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| Affiliation: | LIANG Kang-jian1,BAI Rui-ying2,SONG Li-jun1,SHEN Yu-dong1,XU Zhen-lin1,WANG Hong1,LEI Hong-tao1,YANG Jin-yi1*(1.Key Laboratory of Food Quality and Safety of Education Department of Guangdong Province/Research Institute of Food Quality and Safety,South China Agricultural University,Guangzhou 510642,2.Department of Physiology and Neurobiology,Xinxiang Medical College,Xinxiang 453003) |
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| Abstract: | An indirect competitive enzyme-linked immunosorbent assay(ciELISA) test kit which was based on diethylstilbestrol(DES) polyclonal antibodies(PAbs) for the determination of DES residue in food of animal origin, and to evaluate its performance. The results showed that PAbs had high affinity and specificity to DES. The antibody had cross reactivity of 2.56% and 6.83% with other two synthetic anabolic HEX and DIEN, respectively, but had hardly cross reactivity with estrogen E2, E1, EST and P4. A standard curve ... |
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| Keywords: | diethylstilbestrol polyclonal antibody ciELISA kit |
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