| 大孔吸附树脂法精制金花茶皂甙的工艺研究 |
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| 引用本文： | 韦璐,杨昌鹏,李小妮,李广兰,王莹,刘志新.大孔吸附树脂法精制金花茶皂甙的工艺研究[J].食品科技,2011(9):213-218. |
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| 作者姓名： | 韦璐 杨昌鹏 李小妮 李广兰 王莹 刘志新 |
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| 作者单位： | 广西农业职业技术学院;广西桂人堂金花茶产业集团股份有限公司; |
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| 基金项目： | 广西农业职业技术学院科学研究与技术开放计划项目(桂农职科攻090306) |
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| 摘 要： | 研究大孔吸附树脂法精制金花茶皂甙的工艺条件。比较了聚酰胺、AB-8、XAD-1600和XAD-164种树脂对金花茶皂甙的静态吸附及解吸性能,筛选出最佳吸附剂,并对其吸附动力学特征及解吸条件进行研究。结果表明,XAD-16型树脂的吸附与解吸效果最佳,优化出的最佳柱分离条件为:上样液浓度24.42mg/mL,pH4.88,上样流速1.20BV/h,洗脱过程用1.25BV的蒸馏水除杂后依次用1.75BV30%(v:v),2BV60%(v:v)的乙醇溶液阶段洗脱,精制得2个皂甙组分S2、S3,测定纯度分别为89.48%、70.61%,得率分别为38.94%、30.07%。经颜色反应,两者均为三萜类皂甙。
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| 关 键 词： | 大孔吸附树脂法 金花茶皂甙 精制工艺 |
Purification technology of saponins from Camellia Chrysantha (Hu) Tuyama by macroporous resin |
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| WEI Lu,YANG Chang-peng,LI Xiao-ni,LI Guang-lan,WANG Ying,LIU Zhi-xin.Purification technology of saponins from Camellia Chrysantha (Hu) Tuyama by macroporous resin[J].Food Science and Technology,2011(9):213-218. |
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| Authors: | WEI Lu YANG Chang-peng LI Xiao-ni LI Guang-lan WANG Ying LIU Zhi-xin |
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| Affiliation: | WEI Lu1,YANG Chang-peng1,LI Xiao-ni1,LI Guang-lan1,WANG Ying1,LIU Zhi-xin2 (1.Guangxi Agricultural Vocation-Technology College,Nanning 530007,2.Guangxi GuiRen Golden Camellia Tea Industry Group,Fangchenggang 538021) |
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| Abstract: | To study the purification technology of saponins from Camellia Chrysantha (Hu) Tuyama by macroporous resin, Four types of macroporous resins including polyamide, AB-8, XAD-1600 and XAD-16 were compared for the differences in static adsorption and desorption performances. XAD-16-type macroporous resin showed optimal adsorption and desorption capacities towards Camellia Chrysantha (Hu) Tuyama saponins. And the optimal dynamic adsorption and desorption parameters for the purification of saponins using XAD-16-t... |
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| Keywords: | macroporous resin Camellia chrysantha(Hu) Tuyama saponins purification technology |
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