| 小麦中富马酸的发现、确证及含量测定 |
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| 引用本文： | 何强,孔祥虹,赵洁,李建华,乐爱山. 小麦中富马酸的发现、确证及含量测定[J]. 食品科技, 2011, 0(9): 318-321,326 |
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| 作者姓名： | 何强 孔祥虹 赵洁 李建华 乐爱山 |
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| 作者单位： | 陕西出入境检验检疫局;西安理工大学理学院; |
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| 基金项目： | 陕西省农业科技攻关项目(2010K01-19-4); 国家质检总局科研项目(2009IK285) |
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| 摘 要： | 利用色谱保留时间、紫外吸收光谱及质谱对首次从小麦中发现的富马酸成分进行了确证,并建立了小麦中富马酸含量的超高效液相-串联质谱检测法和高效液相色谱-二极管阵列检测法。样品采用1%氨水-乙腈(体积比为1:1)提取,稀释后进样测定,超高效液相-串联质谱法采用亲水的HILIC色谱柱(50mm×2.1mm,1.7μm)分离,以乙腈-10mmol/L甲酸铵(体积比为85:15)为流动相,电喷雾负离子检测,多反应检测模式定性和定量;高效液相色谱-二极管阵列检测法采用传统的反相C18色谱柱(4.6mm×250mm,5μm)分离,以50mmol/L磷酸二氢铵(pH2.4)为流动相,在波长210nm处检测。小麦样品中的目标物与富马酸标准品具有相同的色谱保留时间、紫外吸收光谱和质谱,确定该目标物为富马酸,而且不同产地的4批小麦样品均检出富马酸;含量测定时的提取方法回收率及重现性良好,2种检测方法均不存在干扰,线性关系良好。首次从小麦中发现并确证了富马酸成分,建立的2种含量检测方法均能满足检测要求。
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| 关 键 词： | 小麦 富马酸 发现 确证 含量 超高效液相色谱-串联质谱 高效液相色谱 |
Discovery, confirmation and quantitative analysis of fumaric acid in wheat |
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| HE Qiang,KONG Xiang-hong,ZHAO Jie,LI Jian-hua,YUE Ai-shan. Discovery, confirmation and quantitative analysis of fumaric acid in wheat[J]. Food Science and Technology, 2011, 0(9): 318-321,326 |
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| Authors: | HE Qiang KONG Xiang-hong ZHAO Jie LI Jian-hua YUE Ai-shan |
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| Affiliation: | HE Qiang1,KONG Xiang-hong1,ZHAO Jie2,LI Jian-hua1,YUE Ai-shan1 |
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| Abstract: | Chromatographic retention time, UV absorption spectrum and mass spectrometry were used to confirm a new composition as fumaric acid found in wheat, and two quantitative analysis methods of fumaric acid in wheat have been developed. An ultra performance liquid chromatograph-tandem mass spectrometry(UPLC-MS/MS) instrument and a high performance liquid chromatograph-diode array detector(HPLC-DAD) instrument were used for the determination. Sample preparation involves extraction with 1% ammonia-water solution/a... |
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| Keywords: | wheat fumaric acid discovery confirmation quantitative analysis ultra performance liquid chromatography-tandem mass spectrometry high performance liquid chromatography |
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