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| 双孢蘑菇SRAP、ISSR、RAPD标记遗传多样性和菌群分类研究 | |
| 引用本文： | 张静,陈文炳,邵碧英,王泽生,廖剑华,李寿崧,江树勋,.双孢蘑菇SRAP、ISSR、RAPD标记遗传多样性和菌群分类研究[J].中国食品学报,2010,10(6):7-13. |
| 作者姓名： | 张静 陈文炳 邵碧英 王泽生 廖剑华 李寿崧 江树勋 |
| 作者单位： | 1. 福建出入境检验检疫局技术中心,福州350001;福建农林大学福州,350002 2. 福建出入境检验检疫局技术中心,福州,350001 3. 福建省农业科学院,福州,350014 |
| 基金项目： | 国家质量监督检验检疫总局科技项目(2007IK159)资助课题的部分内容 |
| 摘 要： | 通过PCR试验,筛选能扩增清晰稳定的DNA条带的53对SRAP引物、10条ISSR引物和56条RAPD引物。SRAP、ISSR和RAPD 3种DNA分子标记扩增位点的平均多态率分别为70.21%、78.33%、61.94%。SRAP、ISSR和RAPD扩增结果用0与1表示,共获得1 046个位点的42 840个数据。采用组间距离法和Jaccard相似性系数,应用SPSS 11.0 for Windows软件进行聚类分析,构建40个双孢蘑菇样品的遗传关系树状图。当组间距离值取16时,40个双孢蘑菇品种可分为6个类群;当组间距离值取13时,40个双孢蘑菇品种被分为9个类群。 |
| 关 键 词： | 双孢蘑菇 遗传多样性 聚类分析 |
Study on Genetic Diversity and Classification of Strain-Groups in Agaricus Bisporus by SRAP,ISSR,RAPD Markers |
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| Zhang Jing,Chen Wenbing,Shao Biying,Wang Zesheng,Liao Jianhua,Li Shousong,Jiang Shuxun.Study on Genetic Diversity and Classification of Strain-Groups in Agaricus Bisporus by SRAP,ISSR,RAPD Markers[J].Journal of Chinese Institute of Food Science and Technology,2010,10(6):7-13. | |
| Authors: | Zhang Jing Chen Wenbing Shao Biying Wang Zesheng Liao Jianhua Li Shousong Jiang Shuxun |
| Affiliation: | Zhang Jing1,2 Chen Wenbing1 Shao Biying1 Wang Zesheng3 Liao Jianhua3 Li Shousong1 Jiang Shuxun1(1Fujian Entry-Exit Inspection and Quarantine Bureau,Fuzhou 350001 2Fujian Agriculture and Forestry University,Fuzhou 350002 3Fujian Academy of Agricultural Science,Fuzhou 350014) |
| Abstract: | By the selection of PCR testing,53 pairs of SRAP primers,10 ISSR primers and 56 RAPD primers were found to be able to amplify clear and stable DNA bands.The average ratio of polymorphic bands in SRAP,ISSR and RAPD marker were 70.21%,78.33% and 61.94% respectively.41 840 data gotten from clear and stable DNA bands at 1 046 sites amplified by SRAP,ISSR and RAPD,coded by 0 or 1,were used to the cluster analysis by means of the between-groups linkage method and the similarity coefficient of Jaccard in the softw... |
| Keywords: | SRAP ISSR RAPD |
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