多菌种分步固态发酵果渣生产菌体蛋白饲料的工艺优化

实验研究了多菌种分步固态发酵果渣生产菌体蛋白饲料的工艺条件,以果渣为主要原料,麸皮、豆粕、谷糠等为辅料,通过对多菌种(黑曲霉、产朊假丝酵母、热带假丝酵母和酿酒酵母)接种及培养方法、菌液的接种量、发酵的最佳温度、共发酵时间、固液比及物料酸碱度因素的优化研究,获得了优化后的多菌种分步固态发酵果渣生产菌体蛋白饲料生产工艺。通过单因素试验及正交试验,确定最佳发酵条件为固液比60%,初始pH5.5,温度30℃,黑曲霉的接种量为7.50%,复合酵母液的接种量为10%,黑曲霉在发酵初期接入,复合酵母液在发酵24h后接入,共培养时间为72h。在最佳菌种配比和发酵条件下,发酵终产物的粗蛋白质含量从4.97%增加至27.60%,增长了22.63个百分点,达到优质蛋白饲料标准。

Technology optimization of multi-strains multi-step solid-state fermentation of fruit residue to produce cell protein feeds

The condition of multi-strains multi-step solid-state fermentation of fruit residue to produce cell protein feeds was studied. Taking fruit residue as the main raw material, bran, soybean meal, carvings and others as accessories, through the study on multi-strains （Aspergillus niger, Candi- da utilis, Candida tropicalis and Saccharomyces cerevisiae） inoculation and cultivation methods, inoculum, optimum temperature for fermentation, co-fermentation time, solid-liquid ratio and material pH, the optimized multi-strains multi-step solid-state fermentation of fruit residue to produce cell protein feeds production process was obtained. By single factor test and orthogonal test, the optimum fermentation conditions were obtained as fol- lows： material quality/water volume 60%, initial pH 5.5, temperature 30~C, inoculum of Aspergillus niger 7.50%, inoculums of compound 10%, A. niger was inoculated in the early stage of fermentation, compound liquid yeast was inoculated after fermentation for 24h, co-fermentation time 72h. Under the optimized condition, the crude protein in end product increased from 4.97% to 27.6%, increased by 22.63%, which achieved the high qual- ity protein feed standard.