| 一株产菊粉内切酶酵母Kluveromyces NSY5发酵条件优化 |
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| 引用本文： | 段学辉,王志园,李强.一株产菊粉内切酶酵母Kluveromyces NSY5发酵条件优化[J].南昌大学学报(工科版),2010,32(1):14-19. |
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| 作者姓名： | 段学辉 王志园 李强 |
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| 作者单位： | 南昌大学食品科学与技术国家重点实验室; |
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| 基金项目： | 江西省科技攻关资助项目(20041B0103000) |
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| 摘 要： | 在单因素实验基础上,采用Plackett-Burman实验设计对影响KluveromycesNSY5发酵产菊粉内切酶的碳源、氮源、无机盐、发酵时间等8个选择因素进行筛选,结果显示,培养基的菊芋汁浓度、摇瓶装液量和发酵时间对菌株发酵产酶具有显著影响。根据实验结果和经验方法对显著影响因素的优化取值范围进行预估,并选择适当的水平设计,用Box-Behnken中心组合实验和响应面寻优分析找出显著影响因素的极优值,验证实验结果表明,采用优化的菊芋汁浓度3.5%、装液量50 mL/250 mL、发酵时间56 h,KluveromycesNSY5摇瓶发酵液中的菊粉内切酶I/S比值达到18.29,比初始发酵条件下的菊粉内切酶I/S比值12.4提高47.5%,优化预测值和实验验证值的相对误差为0.26%。
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| 关 键 词： | 菊粉内切酶 单因子法 Plackett-Burman实验 Box-Behnken实验 响应面分析 |
Optimization of Fermentation Conditions for Endoinulinase-producing Yeast Kluveromyces NSY5 |
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| DUAN Xue-hui,WANG Zhi-yuan,LI Qiang.Optimization of Fermentation Conditions for Endoinulinase-producing Yeast Kluveromyces NSY5[J].Journal of Nanchang University(Engineering & Technology Edition),2010,32(1):14-19. |
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| Authors: | DUAN Xue-hui WANG Zhi-yuan LI Qiang |
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| Affiliation: | State Key Laboratory of Food Science and Technology/a>;Nanchang University/a>;Nanchang 330047/a>;China |
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| Abstract: | Abstract: On the basis of single-factor experiments,some factors affecting the endoinulinase-producing activity of yeast Kluveromyces NSY5 were evaluated by Plackett-Burman design.The experimental results showed that the medium concentration of jerusalem artichoke juice fraction,loaded liquid quantity and the fermentation time had positively influenced on the endoinulinase-producing activity.A central composite design Box-Behnken was employed to optimize the significant factors screened and the results were... |
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| Keywords: | endoinulinase single factor method Plackett-Burman experiments Box-Behnken experiments response surface analysis |
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