| 高香草酸-H_2O_2-HRP荧光酶联免疫分析测定HRP的研究 |
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| 引用本文： | 张书圣,焦奎,季先锋.高香草酸-H_2O_2-HRP荧光酶联免疫分析测定HRP的研究[J].青岛科技大学学报,1997(4). |
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| 作者姓名： | 张书圣 焦奎 季先锋 |
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| 作者单位： | 青岛化工学院应用化学系!青岛266042 |
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| 基金项目： | 青岛化工学院青年科学基金 |
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| 摘 要： | 提出了高香草酸－H2O2－HRP荧光酶联免疫分析测定HRP的新方法。该法基于HRP催化H2O2氧化高香草酸生成能产生荧光的物质，该物质在Ex＝317nm激发光作用下产生荧光，通过测定在Em＝421nm处的荧光强度，间接测定HRP的含量。在所选定的实验条件下，对HRP测定线性范围为1．0×10（-10）～1．0×10（-8）g／mL，检测限为1．0×10（-10）g／mL。
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| 关 键 词： | 荧光光度法 免疫分析 辣根过氧化物酶 高香草酸 |
Fluorescence Enzyme Linked Immunoassay to Detect HRP Using Homovanillic- H_2O_2-HRP System |
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| Zhang Shusheng, Jiao Kui ,Ji Xianfeng.Fluorescence Enzyme Linked Immunoassay to Detect HRP Using Homovanillic- H_2O_2-HRP System[J].Journal of Qingdao University of Science and Technology:Natutral Science Edition,1997(4). |
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| Authors: | Zhang Shusheng Jiao Kui Ji Xianfeng |
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| Abstract: | A new system for the determination of horse radish peroxidase (HRP) was investigated by fluorescence enzyme linked immunoassay. H2O2 could oxidate homovanillic acid catalyzed by HRP to produce a substance which emitted fluorescence (Ex=317nm, Em=421nm), and so HRP could be detected by measuring the density of fluorescence of the oxidaited product. The linear range of the method to detect HRP is (1.0×10(-10)~ 1.0×10(-8)g/mL,and the detection limit 1.0×10(-10)g/mL. |
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| Keywords: | fluorescence spectrophotometry enzyme immunoassay horse radish peroxidase homovanillic |
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