| Recent developments in fluorescence—based mincroscopy applied in biomedical sciences |
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| 引用本文： | Jean－Pierre Timmermans Dirk Adriaensen.Recent developments in fluorescence—based mincroscopy applied in biomedical sciences[J].电子显微学报,2001,20(1):1-10. |
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| 作者姓名： | Jean－Pierre Timmermans Dirk Adriaensen |
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| 作者单位： | Jean-Pierre Timmermans（Lab. of Cell Biology and
Histology，University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerpen, Belgium）;Dirk Adriaensen（Lab. of Cell Biology and Histology，University of
Antwerp, Groenenborgerlaan 171, B-2020 Antwerpen, Belgium） |
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| 摘 要： | 1.IntroductionOverthelastfourdecades,fluorescencemicroscopyhasexperiencedatremendousinnovat-ingevolutionandisnowadaysawellestablishedtechniqueinmanyareasofbiomedicalre-search.Inaddition,fluorescencemicroscopicalimaginghasprovenanessentialtoolforstudy-ingbothcellstructureanddynamicprocessestakingplacewithinonecellorbetweenneigh-bouringcells.Thepresentshortreviewwillfocusonthemostrecentdevelopmentsinthisfieldofmicroscopy.2.ConventionalfluorescencemicroscopyTheterm“fluorescence”denotestheprope…
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| 关 键 词： | 荧光显微境 生物医学 应用 进展 |
| 文章编号： | 1000-6281(2001)01-0001-10 |
Recent developments in fluorescence-based microscopy applied in biomedical sciences |
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| Jean-Pierre Timmermans,Dirk Adriaensen.Recent developments in fluorescence-based microscopy applied in biomedical sciences[J].Journal of Chinese Electron Microscopy Society,2001,20(1):1-10. |
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| Authors: | Jean-Pierre Timmermans Dirk Adriaensen |
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| Affiliation: | Lab. of Cell Biology and Histology,University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerpen, Belgium |
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| Abstract: | The present short review aims to give an overview of the most recent developments in fluorescence microscopy and its applications in biomedical sciences. Apart from improvements in well established methods based on conventional fluorescence microscopy and confocal microscopy (fluorescence in situ hybridisation (FISH), tyramide signal amplification (TSA) in immunocytochemistry, new fluorophores), more recently introduced techniques like fluorescence resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP), multiphoton microscopy and fluorescence correlation spectroscopy (FCS) will be discussed. |
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