| 高效液相色谱法测定果冻中的合成色素 |
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| 引用本文： | 陈艳,刘思洁,李皓.高效液相色谱法测定果冻中的合成色素[J].食品科学,2007,28(9):487-490. |
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| 作者姓名： | 陈艳 刘思洁 李皓 |
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| 作者单位： | 吉林工程技术师范学院生物与食品工程学院； 吉林省卫生检测检验中心； 吉林工程技术师范学院生物与食品工程学院 吉林长春130052； 吉林长春130062； 吉林长春130052； |
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| 摘 要： | 目的:建立果冻中人工合成色素测定的高效液相色谱法。方法:采用C18色谱柱,以乙腈和乙酸铵为流动相,梯度洗脱,流速为1.0ml/min,检测波长为254nm和300nm,柱温25℃,在此色谱条件下进行检测。结果:该方法各组分检出限为0.07～0.16mg/kg,线性范围为1.0～50.0mg/L,加标回收率为98.5%～100.6%。结论:该方法操作简便,准确,快速,提高了样品检出灵敏度,能够同时检测6种合成色素的含量。
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| 关 键 词： | 果冻 色谱法 液相 合成色素 |
| 文章编号： | 1002-6630（2007）09-0487-04 |
| 修稿时间： | 2007-06-11 |
Determination of Synthetic Colour in Jelly by High Performance Liquid Chromatography |
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| CHEN Yan,LIU Si-jie,LI Hao.Determination of Synthetic Colour in Jelly by High Performance Liquid Chromatography[J].Food Science,2007,28(9):487-490. |
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| Authors: | CHEN Yan LIU Si-jie LI Hao |
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| Affiliation: | 1.College of Biology and Food Engineering,Jilin Tercher’s Institute of Engineering and Technology,Changchun 130052,China;2.Jilin Provincial Center for Sanitary Inspection and Test,Changchun 130062,China |
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| Abstract: | Objective:To establish a high performance liquid chromatography method for determination of 6 kinds of synthetic colour in jelly.Methods:6 kinds of synthetic colour were separated on a C18 column using acetonitrile:ammonium acetate as the mobile phase at a flow rate of 1.0 ml/min,with the column temperature 25℃ and detection wave 254 nm and 300 nm.Results:The detection limits were 0.07～0.16 mg/kg,the calibration curves were liner in the range of 1.0～50.0 mg/L,the precision was not more than 8.5% and the rate of recovery were 98.5% to 100.6%.Conclusion:The method established in the present paper is simple,precise,fast and suitable for determination of 6 kinds of synthetic colour in jelly. |
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| Keywords: | jelly chromatography liquid synthetic colour |
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