甘薯叶柄藤类黄酮的体外抗氧化作用研究

研究薯蔓黄酮(FSPV)的体外抗氧化作用。测定了FSPV对二苯代苦味酰自由基(DPPH)、超氧阴离子自由基(O2·)和羟自由基(·OH)的清除率,同时用硫代巴比妥酸(TBA)法测定其对亚油酸的抗氧化性。实验结果表明:FSPV对DPPH的清除效果良好(IC50=8.60mg/L);对O2·清除能力强于芦丁但弱于VC;对·OH的清除能力强于芦丁和VC;对亚油酸也有很好的抗氧化作用。体外实验证实,FSPV具有较强的抗氧化能力。

Study on in vitro Antioxidant Effects of Polysaccharides from Sweet Potato Leaf, Stalk and Stem

The in vitro antioxidant effects of flavonoids of sweet potato vines (FSPV) were studied. The scavenging capacities of FSPV on 1,1-diphenyl 2-picrylhydrazyl, superoxide and hydroxyl free radical were assayed. Meanwhile, the antioxidant activity on linoleic acid was studied with thio barbituric acid(TBA) method. These results showed, on DPPH free radical, the scavenging effect of FSPV is very good (IC50=8.60mg/L). The scavenging activity on superoxide free radical is stronger than lutin’s, but weakerthan VC’s, and the scavenging activity on hydroxyl free radical is better than lutin’s and VC’s. Moreover, FSPV shows strong antioxidant activities on linoleic acid. So, experiments in vitro indicated that FSPV blings about strong antioxidative effect.