| 聚胺与鲑鱼精DNA相互作用中的盐效应 |
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| 引用本文： | 潘华,张剑,张高勇,孙彦庆.聚胺与鲑鱼精DNA相互作用中的盐效应[J].精细化工,2007,24(9):876-880. |
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| 作者姓名： | 潘华 张剑 张高勇 孙彦庆 |
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| 作者单位： | 中国日用化学工业研究院,表面活性剂国家工程研究中心,山西,太原,030001;无锡杰能科生物工程有限公司,上海,200051;亚太区产品研发中心,广州雅芳制造有限公司,广东,广州,510900 |
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| 摘 要： | 通过浊度、紫外吸收和Zeta电位测定,研究了盐对聚胺与鲑鱼精DNA相互作用的影响。结果表明,加入盐产生屏蔽效应,使体系Zeta电位下降,当c(NaC l)=5 mmol/L时,SPM-DNA体系Zeta电位由0 mV降至-4mV,SPD-DNA体系电位由-2.5 mV降至-4 mV。浊度和紫外吸收结果证明了盐屏蔽效应的存在,并随盐浓度的升高,屏蔽效应增大,且Ca2+、Mg2+的屏蔽作用大于K+、Na+。pH的升高使聚胺-DNA的相互作用减弱。
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| 关 键 词： | 聚胺 DNA 盐 相互作用 |
| 文章编号： | 1003-5214（2007）09-0876-05 |
| 修稿时间： | 2007-02-12 |
Effect of Salt on the Interaction between Polyamine and Salmon Sperm DNA |
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| PAN Hua,ZHANG Jian,ZHANG Gao-yong,SUN Yan-qing.Effect of Salt on the Interaction between Polyamine and Salmon Sperm DNA[J].Fine Chemicals,2007,24(9):876-880. |
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| Authors: | PAN Hua ZHANG Jian ZHANG Gao-yong SUN Yan-qing |
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| Affiliation: | State Center for Surfactant Research and Engineering, China Research Institute of Daily Chemical Industry, Taiyuan 030001, Shanxi , China ;Genencor ( Wuxi;GuangZhou |
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| Abstract: | Effect of salt on the interaction between polyamines(spermine(SPM) and spermidine(SPD)) and salmon sperm DNA was studied by measurement of turbidity,UV absorption and zeta potential.The result of zeta potential showed that the electrostatic screen effect of salts made the zeta potential of polyamine-DNA descend.The zeta potential of SPM-DNA system reduced from 0 mV to4 mV when the concentration of NaCl was 5 mmol/L,and that of SPD-DNA system dropped from-2.5 mV to-4 mV when the concentration of NaCl was 0.2 mmol/L.The effect was also confirmed by UV absorption and turbidity measurement.The higher concentration of the salt,the stronger electrostatic screen effect,and the electrostatic screen effect of Ca2 and Mg2 is stronger than that of K and Na .The interaction between polyamine and DNA will be reduced if the pH of the system rises. |
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| Keywords: | polyamine DNA salt interaction |
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